1887

Abstract

Summary

Five or possibly six different soluble adenovirus type 11 components have been identified. Zonal centrifugation separated, listed in order of decreasing sedimentation rates: (i) a complete haemagglutinin (HA), (2) an incomplete HA plus group-specific complement-fixation (CF) antigen, and (3) components absorbing haemagglutination inhibition (HI) and haemagglutination enhancement (HE) antibody. The complete HA carried toxin activity. The incomplete HA, which also exhibited toxin activity, could be separated from group-specific CF antigen by anion-exchange chromatography. The incomplete HA exhibited haemagglutination activity only in the presence of antibody which presumably interacts with vertex capsomere antigen. Antisera against members of all of Rosen’s subgroups contained HE antibody. The major portion of antigen capable of absorbing HE antibody was associated with incomplete HA, but a part of it appeared in a separate fraction. The incomplete HA also exhibited some capacity to absorb HI antibody.

Treatment with trypsin completely destroyed HE antibody-absorbing antigen and eliminated all toxin activity. Repeated erythrocyte absorptions removed all activities except group-specific CF antigen and some HE antibody-absorbing antigen.

By comparison with data obtained in parallel studies of adenovirus types 3, 4 and 5 it is suggested that the different type 11 components are of the following nature:

The incomplete HA might represent isolated penton components—vertex capsomeres plus projections—and the complete HA symmetrical aggregates of 12 such components. The group-specific CF antigen most likely is carried by non-vertex capsomeres (hexon components). HE antibody-absorbing structures not related to incomplete HA appeared heterogeneous and probably included both intact and fragments of free vertex capsomeres. Slowly sedimenting HI antibody-absorbing components presumably represent fibre components, i.e. isolated vertex projections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-2-1-123
1968-01-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/2/1/JV0020010123.html?itemId=/content/journal/jgv/10.1099/0022-1317-2-1-123&mimeType=html&fmt=ahah

References

  1. Fulton F., Dumbell K. R. 1949; The serological comparison of strains of influenza virus. J. gen. Microbiol 3:97
    [Google Scholar]
  2. Ginsberg H. S., Pereira H. G., Valentine R. C., Wilcox W. C. 1966; A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology 28:782
    [Google Scholar]
  3. Kjellén L. 1961; A study of adenovirus-host cell system by the plaque technique. Virology 14:234
    [Google Scholar]
  4. Norrby E. 1966a; The relationship between the soluble antigens and the virion of adenovirus type 3.1. Morphological characteristics. Virology 28:236
    [Google Scholar]
  5. Norrby E. 1966b; The relationship between the soluble antigens and the virion of adenovirus type 3. II. Identification and characterization of an incomplete HA. Virology 30:608
    [Google Scholar]
  6. Norrby E. 1967; Biological significance of structural adenovirus components. Review for Current Topics of Microbiology and Immunology. In the Press
    [Google Scholar]
  7. Norrby E. 1968; Comparison of soluble components of adenovirus types 3 and 11. J. gen. Virol 2:135
    [Google Scholar]
  8. Norrby E., Wadell G. 1967; Soluble components of adenovirus type 4. Virology 31:592
    [Google Scholar]
  9. Norrby E., Skaaret P. 1967; The relationship between the soluble antigens and the virion of adenovirus type 3. III. Immunological identification of fiber antigen and isolated vertex capsomer antigen. Virology 32:789
    [Google Scholar]
  10. Reed L. J., Muench H. 1938; A simple method of estimating fifty-per-cent end points. Am.J. Hyg 27:793
    [Google Scholar]
  11. Rosen L. 1960; A hemagglutination-inhibition technique for typing adenoviruses. Am. J. Hyg 71:120
    [Google Scholar]
  12. Simon M. 1962; Haemagglutination experiments with certain adenovirus type strains. Acta mic robiol. hung 9:45
    [Google Scholar]
  13. Stöhr M., Wigand R. 1965; Untersuchungen fiber Adenovirus-Hamagglutinine der Gruppe I nach Rosen. Arch. ges. Virusforsch 17:20
    [Google Scholar]
  14. Svedmyr A., Enders J. F., Holloway A. 1952; Complement fixation with Brunhilde and Lansing poliomyelitis viruses propagated in tissue culture. Proc. Soc. exp. Biol. Med 79:296
    [Google Scholar]
  15. Valentine R. C., Pereira H. G. 1965; Antigens and structure of the adenovirus. J. molec. Biol 13:13
    [Google Scholar]
  16. Wadell G., Norrby E., Schönning U. 1967; Ultrastructure of soluble antigens and the virion of adenovirus type 4. Arch. ges. Virusforsch 21:237
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-2-1-123
Loading
/content/journal/jgv/10.1099/0022-1317-2-1-123
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error