1887

Abstract

SUMMARY

Treatment of mouse L 929 cells in cell culture with mouse interferon, prior to infection with reovirus type 3, decreased the amount of virus progeny formed. In infected, interferon-treated cells less reovirus specified double-stranded RNA (ds reoRNA) and single-stranded RNA accumulated than in infected control cells and the rate of ds reoRNA accumulation was inversely related to the concentration of interferon with which the cells were treated prior to infection. Since ds reoRNA can be conveniently assayed, this forms the basis of a fast and simple assay for interferons.

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1973-04-01
2024-04-19
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References

  1. Allen P. T., Giron D. J. 1970; Rapid sensitive assay for interferons based on the inhibition of MM virus nucleic acid synthesis. Applied Microbiology 20:317–322
    [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. 1970; Ribonucleic acid synthesis of vesicular stomatitis virus. II. An RNA polymerase in the virion. Proceedings of the National Academy of Sciences of the United States of America 66:572–576
    [Google Scholar]
  3. Burness A. T. H. 1969; Purification of encephalomyocarditis virus. Journal of General Virology 5:291–303
    [Google Scholar]
  4. De Clercq E., Merigan T. C. 1970; Current concepts of interferon and interferon induction. Annual Review of Medicine 21:17–46
    [Google Scholar]
  5. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science, New York 130:432–437
    [Google Scholar]
  6. Finter N. B. 1966; Interferon assays and standards. In Interferons pp 87–118 Edited by Finter N. B. Philadelphia: W. B. Saunders Co.;
    [Google Scholar]
  7. Gauntt C. J. 1972; Effect of interferon on synthesis of ss RNA in reovirus type 3-infected L cell cultures. Biochemical and Biophysical Research Communications 47:1228–1236
    [Google Scholar]
  8. Geiduschek E. P., Moohr J. W., Weiss S. B. 1962; The secondary structure of complementary RNA. Proceedings of the National Academy of Sciences of the United States of America 48:1078–1086
    [Google Scholar]
  9. Gomatos P. J., Tamm I. 1963; The secondary structure of reovirus RNA. Proceedings of the National Academy of Sciences of the United States of America 49:707–714
    [Google Scholar]
  10. Graziadei Hi W. D., Lengyel P. 1972; Translation of in vitro synthesized reovirus messenger RNAs into proteins of the size of reovirus capsid proteins in mouse L cell extract. Biochemical and Biophysical Research Communications 46:1816–1823
    [Google Scholar]
  11. Joklik W. K. 1970; The molecular biology of reovirus. Journal of Cellular Physiology 76:289–301
    [Google Scholar]
  12. Mclaren C. 1970; Influence of cell age on production and assay of mouse interferon in L-cells. Archiv für die gesamte Virusforschung 32:13–18
    [Google Scholar]
  13. Mcwilliams M., Hnkelstein M. S., Allen P. T., Giron D. J. 1971; Assay of chick interferons by the inhibition of viral ribonucleic acid synthesis. Applied Microbiology 21:959–961
    [Google Scholar]
  14. Miller P. A., Lindsay H. L., Cormier M., Mayberry B. R., Trown P. W. 1970; Rapid semi-automated procedures for assaying antiviral activity. Annals of the New York Academy of Sciences 173:151–159
    [Google Scholar]
  15. Mudd J. A., Summers D. F. 1970; Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology 42:328–340
    [Google Scholar]
  16. Oie H. K., Leh P. C. 1968; Reovirus type 2: production of and sensitivity to interferon in human amnion cells (RA). Proceedings of the Society for Experimental Biology and Medicine 127:1210–1213
    [Google Scholar]
  17. Paucker K., Berman B. J., Golgher R. R., Stancek D. 1970; Purification, characterization and attempts at isotopic labeling of mouse interferon. Journal of Virology 5:145–152
    [Google Scholar]
  18. Paucker K., Stanček D. 1972; Characterization of interferon-associated proteins. Journal of General Virology 15:129–138
    [Google Scholar]
  19. Shatkin A. J. 1969; Replication of reovirus. Advances in Virus Research 15:63–87
    [Google Scholar]
  20. Smith A. E., Marcker K. A., Mathews M. B. 1970; Translation of RNA from encephalomyocarditis virus in a mammalian cell-free system. Nature, London 225:184–187
    [Google Scholar]
  21. Stanček D., Paucker K. 1970; Electrophoretic studies with L cell interferon in polyacrylamide gels. Acta virologica 14:125–132
    [Google Scholar]
  22. Stern R., Friedman R. M. 1971; Ribonucleic acid synthesis in animal cells in the presence of actinomycin. Biochemistry 10:3635–3645
    [Google Scholar]
  23. Watanabe Y., Millward S., Graham A. F. 1968; Regulation of transcription of the reovirus genome. Journal of Molecular Biology 36:107–123
    [Google Scholar]
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