Vaccinia virus-induced antigens and polypeptides labelled late in infection with [C]-leucine were examined in gel precipitin tests and polyacrylamide gel electrophoresis followed by autoradiography. Pulse-chase experiments, investigations on the effects of rifampicin and direct analysis of precipitin lines by gel electrophoresis allowed the identification of two major precipitin lines in terms of the polypeptides specific for the infected cell. One of these was non-structural. A small proportion of the other antigen was incorporated into sedimentable structures during a chase; this was prevented by rifampicin. Evidence suggesting that three virus-specified polypeptides are formed by cleavage of larger precursors is also presented and discussed.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error