@article{mbs:/content/journal/jgv/10.1099/0022-1317-18-3-381, author = "Katz, E. and Margalith, Eva", title = "Location of Vaccinia Virus Structural Polypeptides on the Surface of the Virus Particle", journal= "Journal of General Virology", year = "1973", volume = "18", number = "3", pages = "381-384", doi = "https://doi.org/10.1099/0022-1317-18-3-381", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-18-3-381", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Vaccinia virus has a complex structure. Thin sections of virus particles show three structural components: the outer envelope, the lateral bodies and a central structure, known as the ‘core’, which contains the virus DNA (Dales, 1963). Easterbrook (1966) showed that treatment with the nonionic detergent NP40, in the presence of 2-mercaptoethanol (2 ME) leads to the dissociation of the outer membrane and release of the core. By polyacrylamide gel electrophoresis, Holowczak & Joklik (1967) identified at least 17 structural polypeptides, three of which were associated with the core. Katz & Moss (1970) showed that some of these polypeptide peaks could be further separated into two to three polypeptides, thus increasing the total number of resolved structural polypeptides to 22.", }