1887

Abstract

We showed recently that the specific binding of [I]-labelled antivaccinia IgG to the surface of vaccinia-infected cells could be used to detect vaccinia antigens and measure antivaccinia antibody (Hayashi, Rosenthal & Notkins, 1972). The present study shows that this technique can be applied to other viruses and that purified antivirus antibody can be obtained by eluting the specifically-bound immunoglobulins from the surface of infected cells.

Primary rabbit kidney (PRK) cells were prepared as described previously (Hampar 1968). Herpes simplex virus (HSV), type 1, and vaccinia virus, strain 1–79, were propagated and assayed in PRK cells (Hampar 1968; Kempe 1968). Influenza virus, strain -8, was grown in 10-day-old embryonated hen's eggs and assayed by haemagglutination (Davenport & Minuse, 1964). Hyperimmune antisera to each virus were prepared in rabbits (Brier 1971). The gamma globulins were obtained by ammonium sulphate precipitation and fractionated on Sephadex G-200 (Hampar 1968).

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/content/journal/jgv/10.1099/0022-1317-18-2-195
1973-02-01
2019-10-15
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