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Mycoplasmatales virus-laidlawii 2 (MV-L2) was purified by ammonium sulphate precipitation and by sucrose density gradient fractionation. Electron micrographs of virus showed predominantly spherical enveloped particles with a mean diameter of about 80 nm (range 52 to 125 nm). The envelope had a ‘unit membrane’ structure and was probably serologically dissimilar to the ‘unit membrane’ of the host Acholeplasma laidlawii. No obvious isometric or helical capsid was observed within the envelope. The nucleic acid appeared to be DNA.
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