Antisera produced in rabbits against extracts of cells infected with type 1 or type 2 herpes simplex virus neutralized thymidine kinase activities of six type 1 and six type 2 herpes virus strains. There was intertypic neutralizing activity, suggesting some common antigenic determinants, but much greater intratypic activity, suggesting that the enzyme bears type-specific determinants.
The effects of some anti-type 1 sera were complicated since they also stabilized the type 2 enzyme activity.
When anti-type 1 and anti-type 2 sera were absorbed with extracts of cells infected with thymidine-kinase deficient mutants of homologous type they retained their power to neutralize thymidine kinase activity but completely lost their ability to neutralize infectivity. Each absorbed serum gave a single precipitin line against the parent strain and none against the parent of the other type. It was surmised that these precipitin lines were associated with type-specific thymidine kinase antigens.
BuchanA.,
LuffS.,
WallisC.1970; Failure to demonstrate the interaction of subunits of thymidine kinase in cells simultaneously infected with herpes virus and a kinaseiess mutant. Journal of General Virology 9:239–242
BuchanA.,
WatsonD. H.1969; The immunological specificity of thymidine kinases in cells infected by viruses of the herpes group. Journal of General Virology 4:461–463
BuchanA.,
WatsonD. H.,
DubbsD. R.,
KitS.1970; Serological study of a mutant of herpes virus unable to stimulate thymidine kinase. Journal of Virology 5:817–818
FigueroaM. E.,
RawlsW. E.1969; Biological markers for differentiation of herpes virus strains of oral and genital origin. Journal of General Virology 4:259–267
KlempererH. G.,
HaynesG. R.,
SheddenW. I. H.,
WatsonD. H.1967; A virus-specific thymidine kinase in BHK 21 cells infected with herpes simplex virus. Virology 31:120–128
OginoT.,
RappF.1971; Differences in thermal stability of deoxythymidine kinase activity in extracts from cells infected with herpes simplex virus type 1 or 2. Virology 46:953–955
ThoulessM. E.,
SkinnerG. R. B.1971; Differences in the properties of thymidine kinase produced in cells infected with type I and type 2 herpes virus. Journal of General Virology 12:195–197