Determinations of the efficiency of the process of mechanical inoculation of tobacco mosaic virus (TMV) have usually been made with local lesion hosts. To our knowledge, the greatest efficiencies were reported by Steere (1955), who found that one lesion was produced for every 50000 characteristic particles in the inoculum, and by Schramm & Engler (1958), who reported that the minimal TMV concentration detectable on the local lesion test plant was 10 to 10 g/ml (about 1500 to 15000 particles/ml). In the latter paper, Schramm & Engler (1958) also reported that 50% infection of cv. Samsun, a plant which reacts with systemic symptoms, could be obtained by rubbing leaves with 5 ml of an inoculum which contained 10 g/ml of TMV (about 7.5 particles/plant). This indicated that the minimal infective dose for systemically infectible hosts might be less than that for local lesion hosts.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error