1887

Abstract

SUMMARY

We have investigated the capacity of the avian adenovirus CELO to induce interferon in primary chicken embryo fibroblasts. The multiplicity of infection appeared to be an important factor in the induction of interferon.

U.v. irradiation of the virus at 1 or 2 × 10 ergs/mm completely inhibited virus replication, but had no effect on interferon synthesis. Interferon production was decreased by 50% when CELO virus was irradiated with a dose of 5 × 10 ergs/mm.

Treatment of the infected cells with cytosine arabinoside inhibited new DNA synthesis and virus replication, but had little effect on interferon production. Cytosine arabinoside, also, did not inhibit interferon production in chicken cells infected with human adenovirus type 7. It can be concluded that at appropriate multiplicities of infection the parental CELO virus was responsible for interferon induction.

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1972-11-01
2021-10-24
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References

  1. Anderson J. P., McCormick K. J., Stenback W. A., El Mishad A. M., Trentin J. J. 1971; Studies Oil an oncogenic avian adenovirus (CELO). I. Proceedings of Society of Experimental Biology and Medicine 137:399–403
    [Google Scholar]
  2. Beladi I., Pusztai R. 1967; Interferon-like substance produced in chick fibroblasts inoculated with human adenoviruses. Zeitschrift fiir Naturforschung 22:165–169
    [Google Scholar]
  3. Colby C., Duesberg P. H. 1969; Double stranded RNA in vaccinia virus infected cells. Nature, London 222:940–944
    [Google Scholar]
  4. De Clercq E., Merigan T. C. 1969; Requirement of a stable secondary structure for the antiviral activity of polynucleotides. Nature, London 222:1148–1152
    [Google Scholar]
  5. Falcoff R., Falcoff E., Catinot L. 1970; Interferon induction by and infectivity of ultraviolet-irradiated double- and multistranded RNA’s. Biochimica et biophysica acta 217:195–198
    [Google Scholar]
  6. Feldman L. A., Rapp F. 1966; Inhibition of adenovirus replication of 1-β-d-arabinofuranosylcytosine. Proceedings of Society of Experimental Biology and Medicine 122:243–247
    [Google Scholar]
  7. Field A. K., Lampson G. P., Tytell A. A., Nemes M. M., Hilleman M. R. 1967b; InduCerS of interferon and host resistance. IV. Double stranded replicative form RNA from E. coli infected with MS2 coliphage. Proceedings of National Academy of Sciences of the United States of America 58:2102–2108
    [Google Scholar]
  8. Field A. K., Tytell A. A., Lampson G. P., Hilleman M. R. 1967a; Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes. Proceedings of the National Academy of Sciences of the United States of America 58:1004–1010
    [Google Scholar]
  9. Green M., Pina M. 1963; Biochemical studies on adenovirus multiplication. IV. Isolation, purification and chemical analysis of adenovirus. Virology 20:199–207
    [Google Scholar]
  10. Ho M., Kohler K. 1967; Studies on human adenoviruses as inducers of interferon in chick cells. Archiv fiir die gesamte Virusforschung 22:69–78
    [Google Scholar]
  11. Ishibashi M. 1970; Isolation of temperature-sensitive conditional lethal mutants of an avian adenovirus (CELO), and localization of viral entigen in cells infected with them. Biken Journal 13:59–62
    [Google Scholar]
  12. Long W. F., Burke D. C. 1971; Interferon production by double-stranded RNA; a comparison of induction by reovirus to that by a synthetic double-stranded polynucleotide. Journal of General Virology 12:1–11
    [Google Scholar]
  13. Markovits P. 1970; Interférence et production d’interférons dans des cellules de poulet infectées par les adénovirus 4, 7, 8, et 18. Annales de l’Institut Pasteur 118:832–838
    [Google Scholar]
  14. Markovits P., Coppey J. 1971; Induction d’interféron dans des cellules de poulet infectées par un virus á ADN. Comptes-rendus de l’Académie des sciences, série D, Paris 273:1076–1079
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  16. Pusztai R., Bélédi I., Bakay M., Mucsi I. 1969; Effect of ultraviolet irradiation and heating on the interferon-inducing capacity of human adenoviruses. Journal of General Virology 4:169–176
    [Google Scholar]
  17. Tytell A. A., Lampson A. A., Field A. K., Hilleman M. R. 1967; Inducers of interferon and host resistance. III. Double-stranded RNA form reovirus type 3 virions. Proceedings of National Academy of Sciences of the United States of America 58:1719–1722
    [Google Scholar]
  18. Yates V. J., Fry D. E. 1957; Observation on a chicken embryo lethal orphan (CELO) virus. American Journal of Veterinary Research 18:657–660
    [Google Scholar]
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