Amino Acid Composition of Polypeptides from Influenza Virus Particles Free

Abstract

SUMMARY

The neuraminidase, the membrane protein, the ribonucleoprotein (nucleocapsid protein) and the light and heavy polypeptide chains of the haemagglutinin subunits were isolated from particles of influenza virus type A (strain ) and type B (strain ). Each of these substances migrated as a single component during electrophoresis on SDS-polyacrylamide gels. The neuraminidase of type A influenza was isolated from the A-A recombinant virus, X-7F. This enzyme contained two polypeptides differing slightly in electrophoretic mobility.

This paper gives the amino acid composition of the individual polypeptides from the type A and type B influenza viruses and the neuraminidase protein from X-7F virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-17-1-61
1972-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/17/1/JV0170010061.html?itemId=/content/journal/jgv/10.1099/0022-1317-17-1-61&mimeType=html&fmt=ahah

References

  1. Burnet F. M., Beveridge W. I. B., Bull D. R., Clark E. 1942; Investigations of an influenza epidemic in military camps in Victoria in May, 1942. Medical Journal of Australia 2:371–376
    [Google Scholar]
  2. Compans R. W., Klenk H. D., Caligujri L. A., Choppin P. W. 1970; Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology 42:880–889
    [Google Scholar]
  3. Francis T. 1940; A new type of virus from epidemic influenza. Science, New York 92:405–408
    [Google Scholar]
  4. Haslam E. A., Hampson A. W., Egan J. A., White D. O. 1970; The polypeptides of influenza virus. II:
    [Google Scholar]
  5. Interpretation Of Gel Electrophoresis Patterns. Virology 42:555–565
    [Google Scholar]
  6. Hoyle L., Davies S. P. 1961; Amino acid composition of the protein components of influenza A virus. Virology 13:53–57
    [Google Scholar]
  7. Kilbourne E. D., Lief F. S., Schulman J. L., Jahiel R. I., Laver W. G. 1967; Antigenic hybrids in influenza viruses and their implications. Perspectives in Virology 5:87–106
    [Google Scholar]
  8. Knight C. A. 1947; Amino acid composition of highly purified particles of influenza A and B. Journal oj Experimental Medicine 86:125–129
    [Google Scholar]
  9. Laver W. G. 1964; Structural studies on the protein subunits from three strains of influenza virus. Journal of Molecular Biology 9:109–124
    [Google Scholar]
  10. Laver W. G. 1969 Purification of influenza virus. In Fundamental Techniques in Virology Edited by Habel K., Salzman N. P. pp. 82–86 New York: Academic Press;
    [Google Scholar]
  11. Laver W. G. 1971; Separation of two polypeptide chains from the haemagglutinin subunit of influenza virus. Virology 45:275–288
    [Google Scholar]
  12. Laver W. G., Valentine R. C. 1969; Morphology of the isolated haemagglutinin and neuraminidase subunits of influenza virus. Virology 38:105–119
    [Google Scholar]
  13. Laver W. G., Wrigley N. G., Pereira H. G. 1969; Removal of pentons from particles of adenovirus type 2. Virology 39:599–605
    [Google Scholar]
  14. Moore S., Spackman D. H., Stein W. H. 1958; Chromatography of amino acids on sulfonated polystyrene resins - an improved system. Analytical Chemistry 30:1185–1190
    [Google Scholar]
  15. Schulze I. T. 1970; The structure of influenza virus. I. The polypeptides of the virion. Virology 42:890–904
    [Google Scholar]
  16. Skehel J. J., Schild G. C. 1971; The polypeptide composition of influenza A viruses. Virology 44:396–408
    [Google Scholar]
  17. Spackman D. H. 1960 Instruction manual and handbook. Beckman/Spinco Model 120 amino acid analyser. Beckman Instruments Inc.; Spinco Division, Palo Alto, California:
    [Google Scholar]
  18. Taylor J. M., Hampson A. W., Layton J. E., White D. O. 1970; The polypeptides of influenza virus. IV. An analysis of nuclear accumulation. Virology 42:744–752
    [Google Scholar]
  19. Webster R. G. 1970; Estimation of the molecular weights of the polypeptide chains from the isolated haemagglutinin and the neuraminidase subunits of influenza viruses. Virology 40:643–654
    [Google Scholar]
  20. Webster R. G., Laver W. G., Kilbourne E. D. 1968; Reactions of antibodies with surface antigens of influenza virus. Journal of General Virology 3:315–326
    [Google Scholar]
  21. White D. O., Taylor J. M., Haslam E. A., Hampson A. W. 1970 The polypeptides of influenza virus and their biosynthesis. In The Biology of Large RNA Viruses Edited by Barry R. D., Mahy B. W. J. pp. 602–618 New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-17-1-61
Loading
/content/journal/jgv/10.1099/0022-1317-17-1-61
Loading

Data & Media loading...

Most cited Most Cited RSS feed