The glycoproteins of cells infected with herpes simplex virus 1 or 2 (HSV-1 or HSV-2) were solubilized with non-ionic detergent NP-40 and electrophoresed in acrylamide gels. At least 12 bands containing virus glycoproteins were formed. The HSV-1 glycoproteins correspond in electrophoretic mobility to structural proteins in the HSV virions and to the virus proteins in plasma membranes of infected cells. All virus glycoproteins were bound to immunoadsorbent columns made by cross-linking either homologous or heterologous antibody. Immunoadsorbent columns readily discriminated between host and virus glycoproteins. The differentiation between HSV-1 and HSV-2 glycoproteins varied; the degree of specificity of the immunoadsorbent gels was related to the specificity exhibited by the antisera in neutralization tests. All immunoadsorbent antibody gels tested bound homologous glycoproteins in proportions similar to those in the input extract. Immunoadsorbent antibody gels of high specificity bound disproportionate amounts of only a few heterologous glycoproteins. Immunoadsorbent antibody gels of low specificity reacted with heterologous glycoprotein preparations as if they were a homologous preparation.
AbodeelyR. A.,
PalmerE.,
LawsonL. A.,
RandallC. C.1971; The proteins of enveloped and de-enveloped equine abortion (herpes) virus and the separated envelope. Virology 44:146–152
AurelianL.,
RoystonI.,
DavisH. J.1970; Antibody to genital herpes simplex virus; association with cervical atypia and carcinoma in situ. Journal of the National Cancer Institute 45:455–464
AvrameasS.,
TernynckT.1969; The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry 6:53–66
BachenheimerS. L.,
KieffE. D.,
LeeL.,
RoizmanB.1972 Comparative studies of DNA’s of Marek’s disease and herpes simplex virus. In Symposium on Oncogenesis and Herpes-Type Viruses Cambridge; England: June 1971 (in the press)
BernsteinM. T.,
StewartJ. A.1971; A method for typing antisera to herpesvirus hominis by indirect hemagglutination-inhibition. Applied Microbiology 21:680–684
DimmockN. J.,
WatsonD. H.1969; Proteins specified by influenza virus in infected cells: analysis of polyacrylamide gel electrophoresis of antigens not present in the virus particle. Journal of General Virology 5:499–509
DowdleW. R.,
NahmiasA. J.,
HarwellR. W.,
PaulsF. P.1967; Association of antigenic type of herpesvirus hominis with site of viral recovery. Journal of Immunology 99:974–980
EjercitoP. M.,
KieffE. D.,
RoizmanB.1968; Characterization of herpes simplex virus strains differing in their effect of social behavior of infected cells. Journal of General Virology 3:357–364
FairbanksG.Jr.,
LevinthalC.,
ReederR. H.1965; Analysis of 14C-labeled proteins by disc electrophoresis. Biochemical and Biophysical Research Communications 20:393–399
FairbanksG.,
SteckT. L.,
WallachD. F. H.1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
FigueroaM. E.,
RawlsW. E.1969; Biological markers for differentiation of herpes-virus strains of oral and genital origin. Journal of General Virology 4:259–267
FuccilloD. A.,
ModerF. L.,
CatalanoL. W.Jr.,
VincentM. M.,
SeverJ. L.1970; Herpesvirus hominis types 1 and 2: a specific microindirect hemagglutination test. Proceedings of the Society for Experimental Biology and Medicine 133:735–739
GederL.,
SkinnerG. R. B.1971; Differentiation between type 1 and type 2 strains of herpes simplex virus by an indirect immunofluorescence technique. Journal of General Virology 12:179–182
HeineJ. W.,
SpearP. G.,
RoizmanB.1972; The proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane. Journal of Virology 9:431–439
KellerJ. M.,
SpearP. G.,
RoizmanB.1970; The proteins specified by herpes simplex virus. III. Viruses differing in their effects on the social behaviour of infected cells specify different membrane glycoproteins. Proceedings of the National Academy of Sciences of the United States of America 65:865–871
KieffE. D.,
BachenheimerS. L.,
RoizmanB.1971; Size, composition and structure of the DNA of subtypes 1 and 2 herpes simplex virus. Journal of Virology 8:125–132
NahmiasA. J.,
ChiangW. T.,
Del BuenoI.,
DuffeyA.1969a; Typing of herpesvirus hominis strains by a direct immunofluorescent technique. Proceedings of the Society for Experimental Biology and Medicine 132:386–390
NahmiasA. J.,
DowdleW. R.,
KramerJ. H.,
LuceC. F.,
MansourS. C.1969b; Antibodies to herpesvirus hominis types 1 and 2 in the rabbit. Journal of Immunology 102:956–962
NahmaisA. J.,
DowdleW. R.,
NaibZ. M.,
HighsmithA.,
HarwellR. W.,
JoseyW. E.1968; Relation of pock size on chorioallantoic membrane to antigenic type of herpesvirus hominis. Proceedings of the Society for Experimental Biology and Medicine 127:1022–1028
NahmiasA. J.,
JoseyW. E.,
NaibZ. M.,
LuceC. F.,
GuestB.1970b; Antibodies to herpesvirus hominis types 1 and 2 in humans. II. Women with cervical cancer. Americal Journal of Epidemiology 91:547–552
NahmiasA. J.,
NaibZ. M.,
JoseyW. E.1970a; Herpesvirus hominis type 2 infection−association with cervical cancer and perinatal disease. Perspectives in Virology 7:73–89
PlummerG.,
WanerJ. L.,
BowlingC. P.1968; Comparative studies of type 1 and type 2 herpes simplex viruses. British Journal of Experimental Pathology 49:202–208
PlummerG.,
WanerJ. L.,
PhuangrabA.,
GoodheartC. R.1970; Type 1 and type 2 herpes simplex viruses: serological and biological differences. Journal of Virology 5:51–59
RoaneP. R.Jr.,
RoizmanB.1964; Studies of the determinant antigens of viable cells. II. Demonstration of altered antigenic reactivity of Hep-2 cells infected with herpes simplex virus. Virology 22:1–8
RoizmanB.1970 Herpesviruses, membranes and the social behaviour of infected cells. In Proceedings of the 3rd International Symposium on Applied and Medical Virology (Fort Lauderdale, Fla.), pp. 37–72 St Louis, Mo.; Warren Green:
RoizmanB.,
FrenkelN.1972Herpesvirus DNA: structure, synthesis and transcriptionProceedings of the 25th M. D. Anderson Hospital and Tumor Institute Symposium on Molecular Studies in Viral NeoplasiaHouston, Texas8-10 March, 1972 (in the press)
RoizmanB.,
MayerM. M.,
RappH. J.1958; Immunochemical studies of poliovirus. III. Further studies on the immunologic and physical properties of poliovirus particles produced in tissue culture. Journal of Immunology 81:419–425
RoizmanB.,
RoaneP. R.Jr.1961; A physical difference between two strains of herpes simplex virus apparent on sedimentation in cesium chloride. Virology 15:75–79
RoizmanB.,
SpringS. B.1967 Alteration in immunologic specificity of cells infected with cytolytic viruses. In Proceedings Conference on Cross Reacting Antigens and Neoantigens Edited by
TrentinJ. J.
pp. 85–96 Baltimore: Williams and Wilkins Co;
RoystonI.,
AurelianL.1970; Immunofluorescent detection of herpesvirus antigens in exfoliated cells from human cervical carcinoma. Proceedings of the National Academy of Science of the United States of America 67:204–212
SchneweisK. E.,
NahmiasA. J.1971; Antigens of herpes simplex virus type 1 and 2: immunodiffusion and inhibition passive hemagglutination studies. Zeitschrfitfiir Immunlogische Forschung 141:471–487
SchwartzJ.,
RoizmanB.1969; Similarities and differences in the development of laboratory strains and freshly isolated strains of herpes simplex virus in Hep-2 cells: electron microscopy. Journal of Virology 4:879–889
SpearP. G.,
RoizmanB.1970; The proteins specified by herpes simplex virus. IV. The site of glycosylation and accumulation of viral membrane proteins. Proceedings of the National Academy of Sciences of the United States of America 66:730–737
SpearP. G.,
RoizmanB.1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. Journal of Virology 9:143–159
TerniM.,
RoizmanB.1970; Variability of herpes simplex virus: isolation of two variants from simultaneous eruptions at different sites. Journal of Infectious Diseases 121:212–216
Zur HausenH.,
Schulte-HolthausenH.1970; Presence of EB virus nucleic acid homology in a ‘virus-free’ line of Burkitt tumour cells. Nature, London 227:245–248
Zur HausenH.,
Schulte-HolthausenH.,
KleinG.,
HenleW.,
HenleG.,
CliffordP.,
SantessonL.1970; EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx. Nature, London 228:1056–1058