1887

Abstract

In homogenates of HeLa cells 10 to 20% of the ribosomes are bound to membranous structures (Attardi, Cravioto & Attardi, 1969; Rosbash & Penman, 1971; Roumiantzeff, Maizel & Summers, 1971). After treatment with sodium deoxycholate (DOC) to dissolve the membranes, some of these ribosomes equilibrate in density gradients of CsCl at the same position as free ribosomes but others are less dense, having a higher ratio of protein to RNA (Rosbash & Penman, 1971). Large polysomes bearing virus RNA appear in cells infected with poliovirus in the presence of actinomycin D (Penman 1963). These polio polysomes are attached to membranes until treated with DOC (Penman, Becker & Darnell, 1964; Caliguiri & Tamm, 1970; Roumiantzeff 1971). We report here studies of their density in CsCl gradients.

HeLa cells grown in suspension (Harris & Watts, 1962) were resuspended in phosphate-buffered saline containing Ca and Mg (Oxoid Ltd.) at 10 cells/ml and kept at 0 °C for 30 min after adding about 50 p.f.u./cell of poliovirus type 1, strain ts (Cooper, Johnson & Garwes, 1966).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-17-1-143
1972-10-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/17/1/JV0170010143.html?itemId=/content/journal/jgv/10.1099/0022-1317-17-1-143&mimeType=html&fmt=ahah

References

  1. Attardi B., Cravioto B., Attardi B. 1969; Membrane-bound ribosomes in HeLa cells. Journal of Molecular Biology 44:47–70
    [Google Scholar]
  2. Baltimore D., Huang A. S. 1968; Isopycnic separation of subcellular components from poliovirusinfected and normal HeLa cells. Science, New York 162:572–574
    [Google Scholar]
  3. Baltimore D., Huang A. S. 1970; Interaction of HeLa cell proteins with RNA. Journal of Molecular Biology 47:263–273
    [Google Scholar]
  4. Brunk C. F., Leick V. 1969; Rapid equilibrium isopycnic CsCl gradients. Biochimica et biophysica acta 179:136–144
    [Google Scholar]
  5. Caliguiri L. A., Tamm I. 1970; The role of cytoplasmic membranes in poliovirus biosynthesis. Virology 42:100–111
    [Google Scholar]
  6. Cooper P. D., Johnson R. T., Garwes D. J. 1966; Physiological characterization of heat-defective temperature-sensitive poliovirus mutants: preliminary classification. Virology 30:638–649
    [Google Scholar]
  7. Fenwick M. L. 1971; The density of ribosomes bearing messenger RNA in phage-infected and normal bacteria. Journal of Cell Science 8:649–658
    [Google Scholar]
  8. Harris H., Watts J. W. 1962; The relationship between nuclear and cytoplasmic ribonucleic acid. Proceedings of the Royal Society B 156:109–121
    [Google Scholar]
  9. Kaufmann R. L., Ginsburg V. 1968; The metabolism of l-fucose by HeLa cells. Experimental Cell Research 50:127–132
    [Google Scholar]
  10. Olsnes S. 1971; The isolation of polysomes from rat liver. Contamination by membrane proteins with high affinity to ribosomes. Biochimica et biophysica acta 232:705–716
    [Google Scholar]
  11. Penman S., Decker Y., Darnell J. E. 1964; A cytoplasmic structure involved in protein synthesis and assembly of poliovirus components. Journal of Molecular Biology 8:541–555
    [Google Scholar]
  12. Penman S., Scherrer K., Becker Y., Darnell J. E. 1963; Polyribosomes in normal and poliovirus-infected HeLa cells and their relationship to messenger RNA. Proceedings of the National Academy of Sciences of the United States of America 49:654–662
    [Google Scholar]
  13. Rosbash M., Penman S. 1971a; Membrane-associated protein synthesis of mammalian cells. I. The two classes of membrane-associated ribosomes. Journal of Molecular Biology 59:227–241
    [Google Scholar]
  14. Rosbash M., Penman S. 1971b; Membrane-associated protein synthesis of mammalian cells. II. Isopycnic separation of membrane-bound polyribosomes. Journal of Molecular Biology 59:243–253
    [Google Scholar]
  15. Roumiantzeff M., Maizel J. V., Summers D. F. 1971; Comparison of polysomal structures of uninfected and poliovirus infected HeLa cells. Virology 44:239–248
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-17-1-143
Loading
/content/journal/jgv/10.1099/0022-1317-17-1-143
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error