@article{mbs:/content/journal/jgv/10.1099/0022-1317-16-3-339, author = "Harrison, B. D. and Murant, A. F. and Mayo, M. A.", title = "Evidence for Two Functional RNA Species in Raspberry Ringspot Virus", journal= "Journal of General Virology", year = "1972", volume = "16", number = "3", pages = "339-348", doi = "https://doi.org/10.1099/0022-1317-16-3-339", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-16-3-339", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Preparations of top (T), middle (M) and bottom (B) component particles were made by centrifuging purified raspberry ringspot virus in sucrose density gradients. Only B particles were infective alone, but infectivity was increased by adding excess M particles to B particles. RNA obtained from purified virus was fractionated by centrifuging in density gradients and by polyacrylamide gel electrophoresis. Preparations of the larger RNA species (RNA-1, mol. wt 2.4 × 106) were infective but those of the smaller species (RNA-2, mol. wt 1.4 × 106) were not; infectivity of RNA-1 was greatly increased by addition of RNA-2. RNA-2 from M and B particles behaved similarly. The effect of RNA-2 was not mimicked by yeast RNA and was abolished by u.v. irradiation. The RNA-1 and RNA-2 of tobacco ringspot virus behaved like those of raspberry ringspot virus in infectivity tests, but no interaction was detected between RNA species from the two viruses. It is suggested that RNA-1 and RNA-2 carry different pieces of genetic information.", }