RT Journal Article SR Electronic(1) A1 Murant, A. F. A1 Mayo, M. A. A1 Harrison, B. D. A1 Goold, R. A.YR 1972 T1 Properties of Virus and RNA Components of Raspberry Ringspot Virus JF Journal of General Virology, VO 16 IS 3 SP 327 OP 338 DO https://doi.org/10.1099/0022-1317-16-3-327 PB Microbiology Society, SN 1465-2099, AB SUMMARY Particles of raspberry ringspot virus were homogeneous in immunoelectrophoresis tests but sedimented as three components, T, M and B, with sedimentation coefficients of 52, 92 and 130s respectively, and containing 0, 30 and about 44% RNA. The three components were serologically indistinguishable. In CsCl gradients, M and B were each homogeneous, with buoyant densities of 1.43 and 1.52 g./cm.3 respectively. Infectivity was associated with B. RNA extracted from the virus preparations was single-stranded and showed about 20% hyperchromicity in 0.1 m-sodium phosphate buffer at pH 7.0, indicating about 40% base-pairing. Analysis of RNA preparations by polyacrylamide gel electrophoresis revealed two predominant RNA species with mol. wt of 2.4 × 106 (RNA-1) or 1.4 × 106 (RNA-2); RNA-2 was indistinguishable in mol. wt from the RNA-2 of tobacco ringspot virus but RNA-1 migrated slightly more slowly than the RNA-1 of tobacco ringspot virus. M particles of raspberry ringspot virus contain one molecule of RNA-2; B particles contain either one molecule of RNA-1 or, probably, two molecules of RNA-2. Whereas B particles of raspberry ringspot virus were apparently homogeneous in CsCl gradients, B particles of tobacco ringspot virus formed two buoyant density classes., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-16-3-327