1887

Abstract

SUMMARY

Particles of raspberry ringspot virus were homogeneous in immunoelectrophoresis tests but sedimented as three components, T, M and B, with sedimentation coefficients of 52, 92 and 130s respectively, and containing 0, 30 and about 44% RNA. The three components were serologically indistinguishable. In CsCl gradients, M and B were each homogeneous, with buoyant densities of 1.43 and 1.52 g./cm. respectively. Infectivity was associated with B. RNA extracted from the virus preparations was single-stranded and showed about 20% hyperchromicity in 0.1 -sodium phosphate buffer at pH 7.0, indicating about 40% base-pairing. Analysis of RNA preparations by polyacrylamide gel electrophoresis revealed two predominant RNA species with mol. wt of 2.4 × 10 (RNA-1) or 1.4 × 10 (RNA-2); RNA-2 was indistinguishable in mol. wt from the RNA-2 of tobacco ringspot virus but RNA-1 migrated slightly more slowly than the RNA-1 of tobacco ringspot virus. M particles of raspberry ringspot virus contain one molecule of RNA-2; B particles contain either one molecule of RNA-1 or, probably, two molecules of RNA-2. Whereas B particles of raspberry ringspot virus were apparently homogeneous in CsCl gradients, B particles of tobacco ringspot virus formed two buoyant density classes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-16-3-327
1972-09-01
2023-02-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/16/3/JV0160030327.html?itemId=/content/journal/jgv/10.1099/0022-1317-16-3-327&mimeType=html&fmt=ahah

References

  1. BANCROFT J. B. 1971; The significance of the multicomponent nature of cowpea chlorotic mottle virus RNA. Virology 45:830
    [Google Scholar]
  2. CADMAN C. H. 1960; Studies on the relationship between soil-borne viruses of the ringspot type occurring in Britain and continental Europe. Virology 11:653
    [Google Scholar]
  3. CADMAN C. H. 1963; Biology of soil-borne viruses. Annual Review of Phytopathology 1:143
    [Google Scholar]
  4. COX R. A. 1966; The secondary structure of ribosomal RNA in solution. Biochemical Journal 98:841
    [Google Scholar]
  5. DIENER T. O., SCHNEIDER I. R. 1966; The two components of tobacco ringspot virus: origin and properties. Virology 29:100
    [Google Scholar]
  6. DOTY P., BOEDTKER H., FRESCO J. R., HASELKORN R., LITT M. 1959; Secondary structure in ribonucleic acids. Proceedings of the National Academy of Sciences of the United States of America 45:482
    [Google Scholar]
  7. DUNEZ J., DELBOS R., DESVIGNES J.-C, MARENAUD C, KUSZALA J., VUITTENEZ A. 1971; Mise en evidence d’un virus de type ring spot sur. Prunus cerasifera. Annales de Phytopathologie, Numero hors serie 117:
    [Google Scholar]
  8. EDELMAN M., VERMA I. M., LITTAUER U. Z. 1970; Mitochondrial ribosomal RNA from Aspergillus nidulans: characterization of a novel molecular species. Journal of Molecular Biology 49:67
    [Google Scholar]
  9. HANSEN A. J., STACE-SMITH R. 1971; Properties of a virus isolated from golden elderberry. Sambucus nigra aurea. Phytopathology 61:1222
    [Google Scholar]
  10. HARRISON B. D. 1958; Further studies on raspberry ringspot and tomato black ring, soil-borne viruses that affect raspberry. Annals of Applied Biology 46:571
    [Google Scholar]
  11. HARRISON B. D., FINCH J. T., GIBBS A. J., HOLLINGS M., SHEPHERD R. J., VALENTA V., WETTER C. 1971; Sixteen groups of plant viruses. Virology 45:356
    [Google Scholar]
  12. HARRISON B. D., MURANT A. F., MAYO M. A. 1972; Evidence for two functional RNA species in raspberry ringspot virus. Journal of General Virology 16:339
    [Google Scholar]
  13. HARRISON B. D., NIXON H. L. 1960; Purification and electron microscopy of three soil-borne plant viruses. Virology 12:104
    [Google Scholar]
  14. JONES A. T., MURANT A. F. 1971; Serological relationship between cherry leaf roll, elm mosaic and golden elderberry viruses. Annals of Applied Biology 69:11
    [Google Scholar]
  15. KIRBY K. S. 1965; Isolation and characterization of ribosomal ribonucleic acid. Biochemical Journal 96:266
    [Google Scholar]
  16. LANE L. C., KAESBERG P. 1971; Multiple genetic components in bromegrass mosaic virus. Nature, New Biology 232:40
    [Google Scholar]
  17. LOENING U. E. 1967; The fractionation of high molecular weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251
    [Google Scholar]
  18. LOENING U. E. 1968; Molecular weights of ribosomal RNA in relation to evolution. Journal of Molecular Biology 38:355
    [Google Scholar]
  19. LOENING U. E. 1969; The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformation. Biochemical Journal 113:131
    [Google Scholar]
  20. LOENING U. E., INGLE J. 1967; Diversity of RNA components in green plant tissues. Nature, London 215:363
    [Google Scholar]
  21. MARKHAM R. 1960; A graphical method for the rapid determination of sedimentation coefficients. Biochemical Journal 77:516
    [Google Scholar]
  22. MATTHEWS R. E. F. 1970 Defective particles, multiparticle and satellite viruses. Plant Virology226 New York and London: Academic Press;
    [Google Scholar]
  23. MAYO M. A., MURANT A. F., HARRISON B. D. 1971; New evidence on the structure of nepoviruses. Journal of General Virology 12:175
    [Google Scholar]
  24. MURANT A. F. 1970; Raspberry ringspot virus. Commonwealth Mycological Institute/Association of Applied Biologists. Descriptions of Plant Viruses 4:4
    [Google Scholar]
  25. REICHMANN M. E. 1965; Determination of ribonucleic acid content of spherical viruses from sedimentation coefficients of full and empty particles. Virology 25:166
    [Google Scholar]
  26. RINGBORG U., DANEHOLT B., EDSTRÖM J. E., EGYHÄZI E., LAMBERT B. 1970; Electrophoretic characterization of nucleolar RNA from Chironomus teutons salivary gland cells. Journal of Molecular Biology 51:327
    [Google Scholar]
  27. SCHNEIDER I. R. 1970; Tobacco ringspot: an anomalous nucleic acid. Annual Report John Innes Institute 1969:53
    [Google Scholar]
  28. STACE-SMITH R. 1966; Purification and properties of tomato ringspot virus and an RNA-deficient component. Virology 29:240
    [Google Scholar]
  29. STACE-SMITH R., REICHMANN M. E., WRIGHT N. S. 1965; Purification and properties of tobacco ringspot virus and two RNA-deficient components. Virology 25:487
    [Google Scholar]
  30. STEERE R. L. 1956; Purification and properties of tobacco ringspot virus. Phytopathology 46:60
    [Google Scholar]
  31. SZYBALSKI W. 1968 Equilibrium sedimentation of viruses, nucleic acids and other macromolecules in density gradients. Fractions 1968115 Beckman Instruments Inc.; California:
    [Google Scholar]
  32. TOMLINSON J. A., SHEPHERD R. J., WALKER J. C. 1959; Purification, properties and serology of cucumber mosaic virus. Phytopathology 49:293
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-16-3-327
Loading
/content/journal/jgv/10.1099/0022-1317-16-3-327
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error