RT Journal Article SR Electronic(1) A1 Docherty, J. J. A1 Mäntyjärvi, R. A. A1 Rapp, F.YR 1972 T1 Mechanism of the Restricted Growth of Herpes Simplex Virus Type 2 in a Hamster Cell Line JF Journal of General Virology, VO 16 IS 3 SP 255 OP 264 DO https://doi.org/10.1099/0022-1317-16-3-255 PB Microbiology Society, SN 1465-2099, AB SUMMARY A hamster cell line (HDC-22) developed in our laboratory was non-permissive for herpes simplex virus type 2 (HSV-2) but not for herpes simplex virus type 1 (HSV-1). The mechanism of resistance to productive infection by HSV-2 was investigated. Adsorption studies revealed that HSV-1 and HSV-2 adsorbed at similar rates to the HDC-22 cells. The HDC-22 cells, when infected with HSV-2, produced minimal amounts of virus-specific antigens which increased slightly after several days. The amount of HSV-2 DNA produced in the HDC-22 cells was always less than when these cells were infected with HSV-1. Cellular DNA synthesis was severely depressed by infection with HSV-1 at a multiplicity of 1 while HSV-2 had no effect at this multiplicity. At a higher multiplicity (of 5), this effect was increased with HSV-1 and noticeable with HSV-2. These studies indicate that the block for infection of the HDC-22 cell line with HSV-2 is after virus adsorption and may involve synthesis of virus DNA., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-16-3-255