1887

Abstract

SUMMARY

Five temperate phages, two of which are defective, and one presumed temperate, phage PS8, isolated from crown gall tissue, were prepared, purified and compared.

The four phages, omega, PS8, PB2A and LV-1, were indistinguishable. They possess a hexagonal head of about 70 nm. with a flexible tail about 200 nm. long. Their DNA had a of 92.7° ± 0.1°, a % GC of 56.7 ± 0.2, a mol. wt of 34.1 × 10 and comprised the four normal bases A, T, G and C. No evidence was found for repetitive units in PB2A. These four genome DNA types gave 100% hybridization (limit of error 6%). The electrophoretic protein profiles on SDS polyacrylamide gels are essentially identical: four major bands with similar mol. wt. About half of the phage protein has a mol. wt of 48,000 (perhaps a major head protein), about one-third has a mol. wt of 30,000, one sixth has a mol. wt of 15,500 and 4% has a mol. wt of 69,000. No omega-type prophage DNA was detectable in the cured bacteria - by DNA:DNA hybridization. The latter bacteria were as pathogenic as the prophage-containing parent strain -, which throws some doubt on the role of the omega phage group in crown gall induction.

The phage-like particle P0362, isolated from the pathogenic 0362 had a 60 to 70 nm. head and a straight 130 nm. tail. Its DNA was double-stranded with a slightly lower of 92.4°, a mol. wt of 25 × 10. The electrophoretic profiles of coat proteins differed from those of the other phages. Its DNA is less than 17% homologous with the omega group. The eventual role of this phage in crown gall induction remains to be established.

The phage-like particle P8149, isolated from the non-pathogenic 8149, was quite different. The bipyramidal head was much smaller (40 nm. diameter) and had a very short bud-like tail. The DNA was double-stranded, with a of 93.9°, a mol. wt of 10 × 10. The electrophoretic profile of the coat proteins differed from those of the other phages. The DNA does not hybridize with the omega group. As this phage was isolated from a non-pathogenic , it probably has no function in crown gall. 8149 is bilysogenic.

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1972-08-01
2021-10-25
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References

  1. ADAMS M. H. 1959 Bacteriophages New York: Interscience Publishers;
    [Google Scholar]
  2. BEARDSLEY R. E. 1955; Phage production by crown-gall bacteria and the formation of plant tumors. American Naturalist 89:175
    [Google Scholar]
  3. BEARDSLEY R. E. 1960; Lysogenicity in Agrobacterium tumefaciens. Journal of Bacteriology 80:180
    [Google Scholar]
  4. BENDICH A. 1957 Methods for characterization of nucleic acids by base composition. Methods in Enzymology 3715 Ed. by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  5. BRAUN A. C. 1947; Thermal studies on the factors responsible for tumor initiation in crown-gall. American Journal of Botany 34:234
    [Google Scholar]
  6. BRAY G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillator counter. Analytical Biochemistry 1:279
    [Google Scholar]
  7. BRITTEN R. J., KOHNE D. E. 1966; Nucleotide repetition in DNA. Carnegie Institution Year Book 65:78
    [Google Scholar]
  8. BRUNNER M., POOTJES C. F. 1969; Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens. Journal of Virology 3:181
    [Google Scholar]
  9. BUCHWALD M., MURIALDO H., SIMINOVITCH L. 1970; The morphogenesis of bacteriophage lambda. II. Identification of the principal structural proteins. Virology 42:390
    [Google Scholar]
  10. BURTON K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315
    [Google Scholar]
  11. CARO L. G. 1965; The molecular weight of lambda DNA. Virology 25:266
    [Google Scholar]
  12. DE LEY J. 1970; Reexamination of the association between melting point, buoyant density and chemical base composition of deoxyribonucleic acid. Journal of Bacteriology 101:738
    [Google Scholar]
  13. DELEY J., CATTOIR H., REYNAERTS A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. European Journal of Biochemistry 12:133
    [Google Scholar]
  14. DE LEY J., TYTGAT R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek Journal of Microbiology 36:461
    [Google Scholar]
  15. DE LEY J., VAN MUYLEM J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek Journal of Microbiology 29:344
    [Google Scholar]
  16. DE ROPP R. S. 1947; The growth-promoting and tumefacient factors of bacteria-free crown-gall tumor tissue. American Journal of Botany 34:248
    [Google Scholar]
  17. D’HERELLE F., PEYRE E. 1927; Contribution à l’etude des tumeurs experimentales. Comptes rendus de l’Academie des Sciences, Paris 185:227
    [Google Scholar]
  18. FREIFELDER D. 1970; Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. Journal of Molecular Biology 54:567
    [Google Scholar]
  19. GILLIS M., DE LEY J., DE CLEENE M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. European Journal of Biochemistry 12:143
    [Google Scholar]
  20. GRIPPO P., IACCARINO N., ROSSI N., SCARANO E. 1965; Thin-layer chromatography of nucleotides, nucleosides and nucleic acid bases. Biochimica et biophysica acta 95:1
    [Google Scholar]
  21. HOLSTERS M. 1970; Een onderzoek over getemperde bakteriofagen bij. Agrobacterium Thesis. Univ. Gent, Belgium
    [Google Scholar]
  22. ISRAILSKY W. P. 1927; Bacteriophagie und Pflanzenkrebs. II. Zentralblatt fur Bakteriologie, Parasitenkunde und Infektionskrankheiten Jena, Abt. II 71:302
    [Google Scholar]
  23. KENT G. C. 1937; Some physical, chemical and biological properties of a specific bacteriophage for. Pseudomonas tumefaciens. Phytopathology 27:871
    [Google Scholar]
  24. KLEIN R. M., BEARDSLEY R. E. 1957; On the role of omega bacteriophage in formation of crown-gall tumor cells. American Naturalist 91:330
    [Google Scholar]
  25. KLEIN R. M., TENENBAUM I. L. 1955; A quantitative bioassay for crown-gall tumor formation. American Journal of Botany 42:709
    [Google Scholar]
  26. KLEINSCHMIDT A. K., LANG D., JACHERTS D., ZAHN R. K. 1962; Darstellung und Längemessungen des gesamten Desoxyribonucleinsaureinhaltes von T2-Bacteriophagen. Biochimica et biophysica acta 61:857
    [Google Scholar]
  27. KORANT B. D., POOTJES C. F. 1970; Physicochemical properties of Agrobacterium tumefaciens phage LV-1 and its DNA. Virology 40:48
    [Google Scholar]
  28. KURKDJIAN A. 1968; Apparition de phages au cours de l’induction de tumeurs du crown-gall. Journal de Microscopie 7:1039
    [Google Scholar]
  29. KURKDJIAN A. 1970; Observations sur la presence des phages dans les plaies infectees par differentes souches d’Agrobacterium tumefaciens (Smith & Town) Conn. Annales de l’Institut Pasteur 118:690
    [Google Scholar]
  30. KURKDJIAN A., BEARDSLEY R. E., MANIGAULT P. 1968; Bacteriophages d’ Agrobacterium tumefaciens. II. Souche B6m lysogène dans les tissues de Pisum sativum L. et effet de la glycine sur la production des phages. Annales de l’Institut Pasteur 114:555
    [Google Scholar]
  31. LEDOUX L., HUART R. 1969; Fate of exogenous bacterial deoxyribonucleic acids in barley seedlings. Journal of Molecular Biology 43:243
    [Google Scholar]
  32. LEFF I., BEARDSLEY R. E. 1970; Action tumorigène de l’acide nucleique d’un bacteriophage dans les cultures de tissu tumoral de tournesol. (Helianthus annus). Comptes rendus de l’Academie des Sciences, Paris 270:2505
    [Google Scholar]
  33. LEGAULT-DEMARE J., DESSEAUX B., HEYMAN T., SEROR S., RESS G. P. 1967; Studies on hybrid molecules of nucleic acids. I. DNA-DNA hybrids on nitrocellulose filters. Biochemical and Biophysical Research Communications 28:550
    [Google Scholar]
  34. LEIGHTON S. B., RUBENSTEIN I. 1969; Calibration of molecular weight scales for DNA. Journal of Molecular Biology 46:313
    [Google Scholar]
  35. MUNCIE J. H., PATEL M. K. 1930; Studies upon a bacteriophage specific for. Pseudomonas tumefaciens. Phytopathology 20:289
    [Google Scholar]
  36. PARSONS C. L., BEARDSLEY R. E. 1968; Bacteriophage activity in homogenate of crown gall tissue. Journal of Virology 2:651
    [Google Scholar]
  37. QUÉTIER F., HUGUET T., GUILLÉ E. 1969; Induction of crown-gall: partial homology between tumor-cell DNA, bacterial DNA and the G +G-rich DNA of stressed normal cells. Biochemical and Biophysical Research Communications 34:128
    [Google Scholar]
  38. RANDERATH K., RANDERATH E. 1967 Thin-layer separation methods for nucleic acid derivates. Methods in Enzymology 12 part A 323 Ed. by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  39. ROUSSAUX J., KURKDJIAN A., BEARDSLEY R. E. 1968; Bacteriophages d’Agrobacterium tumefaciens (Smith and Townsend) Conn. I. Isolement et caractères. Annales de l’Institut Pasteur 114:237
    [Google Scholar]
  40. SCHILPEROORT R. A. 1969; Investigations on plant tumors. Crown gall. Ph.D. Thesis. Univ. Leiden, Netherlands
    [Google Scholar]
  41. SCHILPEROORT R. A., VELDSTRA H., WARNAAR S. O., MULDER G., COHEN J. A. 1967; Formation of complexes between DNA isolated from tobacco crown gall tumors and RNA complementary to Agrobacterium tumefaciens DNA. Biochimica et biophysica acta 145:523
    [Google Scholar]
  42. SRIVASTAVA E. I. S. 1970; DNA-DNA hybridization studies between bacterial DNA, crown gall tumor cell DNA and the normal cell DNA. Life Sciences 9:889
    [Google Scholar]
  43. STONIER T. 1960; Agrobacterium tumefaciens Conn. I. Release of P32 and S35 by labeled bacteria in vitro. Journal of Bacteriology 79:880
    [Google Scholar]
  44. STONIER T., McSHARRY J., SPEITEL T. 1967; Agrobacterium tumefaciens Conn. IV. Bacteriophage PB2X, and its inhibitory effect on tumor induction. Journal of Virology 1:268
    [Google Scholar]
  45. STROUN M., ANKER P., AUDERSET G. 1970; Natural release of nucleic acids from bacteria into plant cells. Nature, London 227:607
    [Google Scholar]
  46. STROUN M., ANKER P., LEDOUX L. 1967; Apparition de DNA de densites differentes chez Solanum lycopersicum Esc. au cours de la periode d’induction d’une tumeur par la bacterie. Agrobacterium tumefaciens. Comptes rendus de l’Academie des Sciences, Paris 264:1342
    [Google Scholar]
  47. THOMAS C. A., ABELSON J. 1966 The isolation and characterization of DNA from bacteriophage. Procedures in Nucleic Acid Research553 Ed. by Cantoni G., Davies D. New York: Harper & Row;
    [Google Scholar]
  48. TOURNEUR J., MOREL G. 1970; Sur la presence de phages dans les tissus de crown-gall cultives in vitro. Comptes rendus de l’Academie des Sciences, Paris 270:2810
    [Google Scholar]
  49. WEBER K., OSBORN M. 1969; The reliability of molecular weight determinations by dodecylsulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406
    [Google Scholar]
  50. WESTMORELAND B. C, SZYBALSKI W., RIS H. 1969; Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy. Science, New York 163:1343
    [Google Scholar]
  51. YAMAMOTO K. R., ALBERTS B. M., BENZINGER R., LAWHORNE L., TREIBER G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734
    [Google Scholar]
  52. ZIMMERER R. P., HAMILTON R. H., POOTJES C. F. 1966; Isolation, and morphology of temperature Agrobacterium tumefaciens bacteriophages. Journal of Bacteriology 92:746
    [Google Scholar]
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