1887

Abstract

Strains of Newcastle disease virus (NDV) induce cell fusion by two different mechanisms. The first, called ‘fusion from without’ (Bratt & Gallaher, 1969), is independent of virus multiplication, can be induced equally well by infective and non-infective virus and does not require host-specific or virus-specific macromolecular synthesis. This type of cell fusion is completed within 1 to 3 hr of infection at high multiplicity and is caused by the direct interaction of the virus envelope with the plasma membrane of the cell. Fusion from without is a laboratory phenomenon and is similar to the rapid cell fusion induced by high multiplicities of Sendai virus, SV, and measles virus (Poste, 1970). The second type of NDV-induced poly-karyocytosis, called ‘fusion from within’, is characterized by cell fusion beginning several hours after infection at moderate or low multiplicity. This type of cell fusion is related to the intracellular growth of virus and requires virus-specific macromolecular synthesis (Reeve & Poste, 1971; Reeve 1971).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-16-1-95
1972-07-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/16/1/JV0160010095.html?itemId=/content/journal/jgv/10.1099/0022-1317-16-1-95&mimeType=html&fmt=ahah

References

  1. BRATT M. A., GALLAHER W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proceedings of the National Academy of Sciences of the United States of America 64:536
    [Google Scholar]
  2. BRATT M. A., GALLAHER W. R. 1970; Comparison of fusion from within and fusion from without by Newcastle disease virus. In Vitro 6:3
    [Google Scholar]
  3. KOHN A. 1965; Polykaryocytosis induced by Newcastle disease virus in monolayers of animal cells. Virology 26:228
    [Google Scholar]
  4. KOHN A., FUCHS P. 1969; Cell fusion by strains of Newcastle disease virus and their virulence. Journal of Virology 3:539
    [Google Scholar]
  5. METHODS FOR THE EXAMINATION OF POULTRY BIOLOGICS 1963 Publication No. 1038 National Academy of Sciences National Research Council; Washington, D.C., U.S.A:
    [Google Scholar]
  6. OKADA Y., MURAYAMA F. 1968; Fusion of cells by HVJ: Requirement of concentration of virus particles at the site of contact of two cells for fusion. Experimental Cell Research 52:34
    [Google Scholar]
  7. POSTE G. 1970; Virus-induced polykaryocytosis and the mechanism of cell fusion. Advances in Virus Research 16:303
    [Google Scholar]
  8. POSTE G., REEVE P. 1971; Formation of hybrid cells and heterokaryons by fusion of enucleated and nucleated cells. Nature New Biology 229:123
    [Google Scholar]
  9. REEVE P., ALEXANDER D. J., POPE G., POSTE G. 1971; Studies on the cytopathic effects of Newcastle disease virus: metabolic requirements. Journal of General Virology 11:25
    [Google Scholar]
  10. REEVE P., POSTE G. 1971a; Cell fusion by Newcastle disease virus in the absence of RNA synthesis. Nature New Biology 229:157
    [Google Scholar]
  11. REEVE P., POSTE G. 1971b; Studies on the cytopathogenicity of Newcastle disease virus: relation between virulence, polykaryocytosis and plaque size. Journal of General Virology 11:17
    [Google Scholar]
  12. REEVE P., POSTE G., ALEXANDER D. J., POPE G. 1972; Studies on the cytopathic effects of Newcastle disease virus: cell surface changes. Journal of General Virology 15:219
    [Google Scholar]
  13. REEVE P., ROSENBLUM M., ALEXANDER D. J. 1970; Growth in chick chorioallantoic membranes of strains of Newcastle disease virus of differing virulence. Journal of Hygiene, Cambridge 68:61
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-16-1-95
Loading
/content/journal/jgv/10.1099/0022-1317-16-1-95
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error