The rates of synthesis and final yields of poliovirus RNA varied considerably during the four main phases (G1, S, G2 and m) of the life cycle of synchronized HeLa cells. The rate of RNA synthesis late in virus growth (as measured by uridine incorporation 2 to 4 hr after infection) and the final yield of RNA rose sharply if growth was initiated towards the end of phase S; that is, RNA was synthesized most rapidly if cells were infected during the period of most rapid DNA synthesis. In contrast, the initial rate of RNA synthesis (incorporation 0 to 2 hr after infection) was greatest if growth was initiated at the end of phase G2, just before mitosis. This differential effect on growth kinetics suggests that the balance between the two stages of virus RNA synthesis (production of complementary minus strands and of progeny plus strands) is dependent on unknown cellular factors.
COOPERP. D., WENTWORTHB. B., McCAHOND.1970; Guanidine inhibition of poliovirus: a dependence of viral RNA synthesis on the configuration of structural protein. Virology 40:486
DARNELLJ. E., GIRARDM., BALTIMORED., SUMMERSD. F., MAIZELJ. V.1967 The synthesis and translation of poliovirus RNA. The Molecular Biology of Viruses375–401 New York: Academic Press;
DJACZENKOW., BENEDETTOA., PEZZIR.1970; Formation of helical polyribosomes in poliovirus-infected cells of the 37 RC line. The Journal of Cell Biology 45:173
KESEROVICB.1971; Dejstvo herpes simplex virusa na deobu L celija. International Symposium on Experimental Oncology3rd Yugoslavian Congress of Cancerology Zagreb, Abstracts IV:439
LAWRENCEW. C.1971; Evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the S phase of the KB cell mitotic cycle. Journal of Virology 7:736
LEVINTOWL., DARNELLJ. E.1960; A simplified procedure for purification of large amounts of poliovirus: characterization and amino acid analysis of type 1 poliovirus. Journal of Biological Chemistry 235:70
SALBJ. M., MARCUSP. I.1965; Translational inhibition in mitotic HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:1353
VOLPEP., EREMENKOT.1971 Structural enzyme heterogeneity as expressed by differentiated regulation during the phases of the cell cycle. Advances in Cytopharmacology1257 New York: Raven Press;