1887

Abstract

SUMMARY

The basis for accumulation of capsid antigen in the cytoplasm of cells infected with a newly recognized variant of polyoma has been investigated. The virus progeny and the infectious cycle were compared with that of a normal large plaque strain (lp-s). No significant differences to account for the variation were observed. Expression of this phenotypic character was modified by reduced temperature of incubation combined with serum or arginine deprivation or independently by treatment of infected cells at specific times post-infection with 5-fluorodeoxyuridine actinomycin D or Actidione (cycloheximide).

These results are compatible with the hypothesis that accumulation of capsid antigen in the cytoplasm results from its synthesis at a rate greater than its diffusion into the nucleus. The molecular basis of the defect is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-16-1-29
1972-07-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/16/1/JV0160010029.html?itemId=/content/journal/jgv/10.1099/0022-1317-16-1-29&mimeType=html&fmt=ahah

References

  1. BERECZKY E., HUGHES R., BOWEN J. M., MUNYON W., DMOCHOWSKY L. 1965; Study of DNA synthesis and antigen formation in polyoma virus infected mouse embryo cells by autoradiography and immunofluorescence. Texas Reports on Biology and Medicine 23:3
    [Google Scholar]
  2. BOURGAUX P., BOURGAUX-RAMOISY D., DULBECCO R. 1969; The replication of the ring shaped DNA of polyoma virus. I. Identification of the replicative intermediate. Proceedings of the National Academy of Sciences of the United States of America 64:701
    [Google Scholar]
  3. BUTEL J. S., GUENTZEL M. J., RAPP F. 1969; Variants of defective simian papovavirus 40 (PARA) characterized by cytoplasmic localization of simian papovavirus 40 tumor antigen. Journal of Virology 4:632
    [Google Scholar]
  4. DALTON A. J. 1955; A chrome osmium fixative for electron microscopy. Anatomical Record 121:281
    [Google Scholar]
  5. DIAMOND L., CRAWFORD L. V. 1964; Some characteristics of large plaque and small plaque lines of polyoma virus. Virology 22:235
    [Google Scholar]
  6. DULBECCO R., FREEMAN G. 1959; Plaque production by the polyoma virus. Virology 8:396
    [Google Scholar]
  7. HARE J. D. 1967; Location and characterization of the phenylalanine transport mechanism in normal and polyoma-transformed hamster cells. Cancer Research 27:2357
    [Google Scholar]
  8. HARE J. D. 1970; A new type of variation among the polyoma viruses characterized by cytoplasmic accumulation of capsid antigen. Virology 40:978
    [Google Scholar]
  9. HARE J. D., BALDUZZI P., MORGAN H. R. 1963; Polyoma virus and L cell relationship. I. Some characteristics of a cell line persistently infected with polyoma virus. Journal of the National Cancer Institute 30:45
    [Google Scholar]
  10. HARE J. D., CHAN J. C. 1966; Antigenic variation among polyoma viruses from different sources demonstrated by plaque neutralization. Virology 30:62
    [Google Scholar]
  11. HARE J. D., CHAN J. E. 1970; Studies on the immunochemical basis for antigenic variation among polyoma virus strains. Journal of the National Cancer Institute 45:1179
    [Google Scholar]
  12. HENLE G., DEINHARDT F., RODRIGUEZ J. 1959; The development of polyoma virus in mouse embryo cells as revealed by the fluorescent antibody staining. Virology 8:388
    [Google Scholar]
  13. HIRT B. 1967; Selective extraction of polyoma virus DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365
    [Google Scholar]
  14. ISHIBASHI M. 1970; Retention of viral antigen in the cytoplasm of cells infected with temperature-sensitive mutants of an avian adenovirus. Proceedings of the National Academy of Sciences of the United States of America 65:304
    [Google Scholar]
  15. MALMGREN R. A., RABATTI R., RABSON A. S. 1960; Intracellular localization of polyoma virus antigen demonstrated with fluorescein-labelled antisera. Journal of the National Cancer Institute 24:581
    [Google Scholar]
  16. McCULLOCH E. A., HOWATSON A. F., SIMINOVITCH L., AXELRAD A. A., HAM A. W. 1959; Tumor induction in Swiss mice by virus obtained originally from a mammary tumor in C3H/Jax mouse and then cultivated in vitro. Proceedings of American Association for Cancer Research 3:41
    [Google Scholar]
  17. McCUTCHAN J. H., PAGANO J. S. 1968; Enhancement of the infectivity of simian virus 40 desoxyribonucleic acid with diethyl aminoethyldextran. Journal of the National Cancer Institute 41:351
    [Google Scholar]
  18. SACHS L., FOGEL M. 1960; Polyoma virus synthesis in tumor cells as measured by the fluorescent antibody technique. Virology 11:722
    [Google Scholar]
  19. VELICER L. F., GINSBERG H. S. 1969; Cytoplasmic synthesis of type 5 adenovirus capsid proteins. Proceedings of the National Academy of Sciences of the United States of America 61:1265
    [Google Scholar]
  20. WILLIAMS M. G., SHEININ R. 1961; Cytological studies of mouse embryo cells infected with polyoma virus, using acridine orange and fluorescent antibody. Virology 13:368
    [Google Scholar]
  21. WINOCOUR E. 1963; Purification of polyoma virus. Virology 19:158
    [Google Scholar]
  22. WINTERS A. L., CONSIGLI R. A. 1971; Effects of arginine deprivation on polyoma virus infection of mouse embryo cultures. Journal of General Virology 10:53
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-16-1-29
Loading
/content/journal/jgv/10.1099/0022-1317-16-1-29
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error