1887

Abstract

SUMMARY

The molecular size of the structural proteins of the Indiana and New Jersey serotypes of vesicular stomatitis virus (VSV) and of Cocal virus have been compared by co-electrophoresis in SDS-polyacrylamide gel. Virus polypeptides (VP) II, III and V of the Indiana serotype have different electrophoretic mobilities from the corresponding components of the New Jersey serotype. Cocal virus differs from VSV Indiana in the mobility of VP II and VP V, and from VSV New Jersey in the mobility of VP II and VP III. Mol. wt for VP II, III, IV and V of all three viruses have been determined by co-electrophoresis with labelled radioactive protein markers. labelling of VSV polypeptides did not affect their electrophoretic mobility.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-16-1-1
1972-07-01
2024-10-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/16/1/JV0160010001.html?itemId=/content/journal/jgv/10.1099/0022-1317-16-1-1&mimeType=html&fmt=ahah

References

  1. BURGE B. W., HUANG A. S. 1970; Comparisons of membrane protein glycopeptides of Sindbis virus and vesicular stomatitis virus. Journal of Virology 6:176
    [Google Scholar]
  2. CARTWRIGHT B., SMALE C. J., BROWN F. 1969; Surface structure of vesicular stomatitis virus. Journal of General Virology 5:1
    [Google Scholar]
  3. CARTWRIGHT B., TALBOT P., BROWN F. 1970; The proteins of biologically active sub-units of vesicular stomatitis virus. Journal of General Virology 7:267
    [Google Scholar]
  4. FEDERER K. E., BURROWS R., BROOKSBY J. B. 1967; Vesicular stomatitis virus - the relationship between some strains of the Indiana serotype. Research in Veterinary Science 8:103
    [Google Scholar]
  5. HELLEINER C. W., WUNNER W. H. 1971; A simple method for counting 14C- and 3H-proteins in Polyacrylamide gels. Analytical Biochemistry 39:333
    [Google Scholar]
  6. JONKERS A. H., SHOPE R. E., AITKEN T. H. G., SPENCE L. 1964; Cocal virus, a new agent in Trinidad related to vesicular stomatitis virus, type Indiana. American Journal of Veterinary Research 25:236
    [Google Scholar]
  7. KANG C. Y., PREVEC L. 1970; Proteins of vesicular stomatitis virus. II. Immunological comparisons of viral antigens. Journal of Virology 6:20
    [Google Scholar]
  8. KLOTZ I. M., DARNALL D.W. 1969; Protein subunits ratable (2nd edition). Science, New York 166:126
    [Google Scholar]
  9. MacPherson I. A., STOKER M. G. P. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  10. MUDD J. A., SUMMERS D. F. 1970; Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology 42:328
    [Google Scholar]
  11. PRINGLE C. R. 1970a The induction and genetic characterization of conditional lethal mutants of vesicular stomatitis virus. The Biology of large RNA viruses Ed. by Barry R. D., Mahy B. W. J.567–82 New York: Academic Press Inc;
    [Google Scholar]
  12. PRINGLE C.R. 1970b; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine and ethyl methane sulfonate. Journal of Virology 5:559
    [Google Scholar]
  13. PRINGLE C. R., DUNCAN I. B., STEVENSON M. 1971; Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus. New Jersey serotype. Journal of Virology 8:836
    [Google Scholar]
  14. RICE R. H., MEANS G. E. 1971; Radioactive labelling of proteins in vitro. Journal of Biological Chemistry 246:831
    [Google Scholar]
  15. SHAPIRO A. L., VINUELA E., MAIZEL J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815
    [Google Scholar]
  16. WAGNER R. R., SCHNAITMAN T. A., SNYDER R. M. 1969; Structural proteins of vesicular stomatitis viruses. Journal of Virology 3:395
    [Google Scholar]
  17. WAGNER R. R., SNYDER R. M., YAMAZAKI S. 1970; Proteins of vesicular stomatitis virus: Kinetics and cellular sites of synthesis. Journal of Virology 5:548
    [Google Scholar]
  18. WEBER K., OSBORN M. 1969; The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406
    [Google Scholar]
  19. WUNNER W. H., PRINGLE C. R. 1972; Protein synthesis in BHK-21 cells infected with vesicular stomatitis virus. I. ts mutants of the Indiana serotype. Virology 48:104
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-16-1-1
Loading
/content/journal/jgv/10.1099/0022-1317-16-1-1
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error