1887

Abstract

Summary

The morphology of Sendai virus envelope is described in detail. The interaction of the virus envelope and erythrocytes is examined by negative staining and ultrathin sectioning.

In this morphological study haemolysis is seen to progress in the following sequence: attachment of virus to cell membrane; dissolution of cell membrane at the site of attachment; dissolution of spikes opposite the dissolved cell membrane; fusion of the innermost virus (nanogranular) layer with the cell membrane proper; extension of this fused area with annular formation and extrusion of the virus internal components into the lumen of the cell; finally integration of the virus envelope into the cell envelope and detachment of the spikes. This stage could then lead to haemolysis and fusion. Haemolysis could occur through breaks in the unstable virus part of the integrated membrane and cell fusion could occur when this area is stabilized. Cell fusion of two or more cells is achieved by bridging with interspersed virus envelopes.

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/content/journal/jgv/10.1099/0022-1317-15-3-227
1972-06-01
2024-07-15
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References

  1. Apostolov K., Flewett T. H. 1969; Further observations on the structure of influenza viruses A and C. Journal of General Virology 4:365
    [Google Scholar]
  2. Hosaka Y., Koshi Y. 1968; Electron microscopic study of cell fusion by H. V. J. virions. Virology 34:419
    [Google Scholar]
  3. Howe C., Morgan C. 1969; Interactions between Sendai virus and human erythrocytes. Journal of Virology 3:70
    [Google Scholar]
  4. Humphrey J. H., Dourmashkin R. R. 1965; Electron microscope studies of immune cell lysis. Complement C.I.B.A. Foundation Symposium pp 175–186
    [Google Scholar]
  5. Okada Y. 1958; The fusion of Ehrlich’s tumor cells caused by H,. V,. J. virus in vitro. Biken Journal 1:103
    [Google Scholar]
  6. Poste G. 1970; Virus-induced polykaryocytosis and the mechanism of cell fusion. Advances in Virus Research 16:303
    [Google Scholar]
  7. Schneeberger E. E., Harris H. 1966; An ultrastructural study of interspecific cell fusion induced by inactivated Sendai virus. Journal of Cell Science 1:401
    [Google Scholar]
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