We have investigated the effect of ethidium bromide (EB) on synthesis of nuclear, virus and mitochondrial DNA in permissive cells infected with simian virus 40 (SV 40). In the presence of EB, no newly formed, closed-circular, mitochondrial DNA (M-DNA) could be detected by isopycnic EB-CsCl gradient centrifugation. Synthesis of nuclear and virus DNA was not inhibited by a concentration of 8 µg./ml., even though SV40-DNA and M-DNA are similar in their tertiary structure, in that both are supercoiled, closed-circular, double-stranded molecules. The yield of SV40 progeny under these conditions was not reduced nor was their specific infectivity affected, as compared to control values.
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