The proteins from bacteriophage Ø29 have been dissociated with 1% SDS and 2% 2-mercaptoethanol and have been examined by SDS-acrylamide gel electrophoresis. Seven different polypeptides bands have been detected in the purified virus. One of the bands (b1) is frequently detected in different positions suggesting that it represents an aggregate rather than a very large polypeptide. The molecular weights of the other polypeptide bands (b2, b3, b3–4, b4, b5 and b6) estimated by co-electrophoresis with marker proteins are 93,000; 75,000; 65,000; 53,000; 41,000 and 30,000. Protein bands b2, b4 and b6 compose 80% of the bacteriophage. The six structural polypeptides described here could account for approximately 60% of the information contained in the virus DNA.
AndersonD. L.,
HickmanD. D.,
ReillyB. E.1966; Structure of Bacillus subtilis bacteriophage Ø29 and the length of Ø29 deoxyribonucleic acid. Journal of Bacteriology 91:2081
BachrachU.,
ReuvenL.,
FriedmannA.1970; Studies on phage internal proteins; Isolation of protein DNA complexes from T 2 phages and from phage-infected bacteria. Virology 40:882
HagenE. W.,
ZeeceU. M.,
AndersonD. L.1971; A genetic study of temperature-sensitive mutants of the Bacillus subtilis bacteriophage Ø29. Virology 43:561
JovinT.,
ChrambachA.,
NaughtonM. A.1964; An apparatus for preparative temperature-regulated polyacrylamide gel electrophoresis. Analytical Biochemistry 9:351
ShapiroA. L.,
VinuelaE.,
MaizelJ. V.1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815
SpizizenJ.1958; Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proceedings of the National Academy of Sciences 44:1072
WeberK.,
OsbornM.1969; The reliability of molecular weight determination by dodecyl-sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406