Skip to content
1887

Abstract

SUMMARY

The two species of RNA have been purified from the long and short particles of the multicomponent plant virus, tobacco rattle isolate CAM. 3′-End-group analysis, using the technique of periodate oxidation followed by reduction with [H]-borohydride, showed that both species of RNA had an unphosphorylated cytidine residue at the 3′ end.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-14-2-199
1972-02-01
2025-03-23
Loading full text...

Full text loading...

References

  1. Caspar D. L. D. 1963; Assembly and stability of the tobacco mosaic virus particle. Advances in Protein Chemistry 18:37
    [Google Scholar]
  2. De wachter R., Fiers W. 1967; Studies on the bacteriophage MS 2 IV. The 3′-OH terminal undecanucleotide sequence of the viral RNA chain. Journal of Molecular Biology 30:507
    [Google Scholar]
  3. Fraenkel-Conrat H. 1967; Preparation and testing of tobacco mosaic virus - RNA. In Procedures in Nucleic Acid Research pp 480–487 Ed. by Cantoni G. L., Davies D. R. New York: Harper and Row;
    [Google Scholar]
  4. Frost R. R., Harrison B. D., Woods R. D. 1967; Apparent symbiotic interaction between particles of tobacco rattle virus. Journal of General Virology 1:57
    [Google Scholar]
  5. Gilham P. T. 1970; RNA sequence analysis. Annual Review of Biochemistry 39:227
    [Google Scholar]
  6. Glitz D. G., Bradley A., Fraenkel-Conrat H. 1968; Nucleotide sequences at the s’-linked ends of viral ribonucleic acids. Biochimica et biophysica acta 161:1
    [Google Scholar]
  7. Godson N., Sinsheimer R. L. 1967; Lysis of Escherichia coli with a neutral detergent. Biochimica et biophysica acta 149:476
    [Google Scholar]
  8. Harrison B. D., Nixon H. L. 1959; Separation and properties of tobacco rattle virus with different lengths. Journal of General Microbiology 21:569
    [Google Scholar]
  9. Harrison B. D., Woods P. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610
    [Google Scholar]
  10. Kassanis B. 1968; Satellism and related phenomena. Advances in Virus Research 13:173
    [Google Scholar]
  11. Lister R. M., Bracker C. E. 1969; Defectiveness and dependence in three related strains of tobacco rattle virus. Virology 37:262
    [Google Scholar]
  12. Patterson M. S., Greene R. C. 1965; Measurement of low energy beta-emitters in aqueous solution by liquid scintillation counting of emulsions. Analytical Chemistry 37:854
    [Google Scholar]
  13. Sanger H. L. 1968; Characteristics of tobacco rattle virus: evidence that its two particles are functionally defective and mutually complementing. Molecular and General Genetics 101:346
    [Google Scholar]
  14. Sinsheimer R. L., Starman B., Nagler C., Guthrie S. 1962; Process of infection with bacteriophage ox 174 I. Evidence for a ‘replicative form’. Journal of Molecular Biology 4:142
    [Google Scholar]
  15. Solymosy F., Fedorksac I., Gulyas A., Farkas G. L., Ehrenberg L. 1968; A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid, from plant tissues. European Journal of Biochemistry 5:520
    [Google Scholar]
  16. Weber H., Weissmann C. 1970; The 3′-termini of bacteriophage Q/9 plus and minus strands. Journal of Molecular Biology 51:215
    [Google Scholar]
  17. Whitfield P. R. 1965; Application of the periodate method for the analysis of nucleotide sequence to tobacco mosaic virus RNA. Biochimica et biophysica acta 108:202
    [Google Scholar]
  18. Wimmer E., Reichmann M. E. 1969; Two 3′-terminal sequences in satellite tobacco necrosis virus RNA. Nature, London 221:1122
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-14-2-199
Loading
/content/journal/jgv/10.1099/0022-1317-14-2-199
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error