1887

Abstract

SUMMARY

A plaque assay is described in which cytolytic activity of lymphocytic choriomeningitis virus (LCMV) is observed at low but not at high concentrations of infecting virus. Quantitation of the interference at high virus concentrations is detailed. Use of this assay during the course of the LCMV infection in L cells has shown that an interfering component is produced in abundance after the initial peak in infectivity in the medium has been reached. Thereafter the ability of the virus stock to form infective centres declines while the interference activity rises. Coinfection of L cells with high concentrations of one LCMV strain and low concentrations of any other tested strain prevents cellular destruction. When small virus inocula are used interference can be observed within single ‘bull’s eye’ plaques which exhibit concentric rings of lysed and intact cells. Simultaneous infection of L cells with an auto-interfering concentration of LCMV does not interfere significantly with plaque formation by mengo, vaccinia, and vesicular stomatitis viruses, but reduces four- to fivefold the number of plaques formed by the Amapari and Parana viruses related to LCVM. Interferon does not play a role in the system described. The physical and the immunological data on the interfering substance are consistent with the hypothesis that defective-interfering virus particles are present in LCMV tissue culture stocks.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-14-2-177
1972-02-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/14/2/JV0140020177.html?itemId=/content/journal/jgv/10.1099/0022-1317-14-2-177&mimeType=html&fmt=ahah

References

  1. Benson L. M. 1961; Autointerference due to lymphocytic choriomeningitis in tissue culture. Annual Report of the Division of Laboratories and Research, New York State Department of Health 46:
    [Google Scholar]
  2. Camyre K. P., Pfau C. J. 1968; Biophysical and biochemical characterization of lymphocytic choriomeningitis virus. IV. Strain differences. Journal of Virology 2:161
    [Google Scholar]
  3. Cooper P. D., Bellett A. J. D. 1959; A transmissible interfering component of vesicular stomatitis virus preparations. Journal of General Microbiology 21:485
    [Google Scholar]
  4. Franklin R. M. 1961; Comparison of assays for mengo virus and reovirus. Proceedings of the Society of Experimental Biology and Medicine 107:651
    [Google Scholar]
  5. Hotchin J., Benson L. 1963; The pathogenesis of lymphocyctic choriomeningitis: the effects of different inoculation routes and the footpad response. Journal of Immunology 91:460
    [Google Scholar]
  6. Hotchin J. E., Cinits M. 1958; Lymphocytic choriomeningitis infection of mice as a model for the study of latent virus infections. Canadian Journal of Microbiology 4:149
    [Google Scholar]
  7. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325
    [Google Scholar]
  8. Lehmann-Grube F. 1967; A carrier state of lymphocytic choriomeningitis in L cell cultures. Nature, London 213:770
    [Google Scholar]
  9. Lehmann-Grube F., Slenczka W., Tees R. 1969; A persistent and inapparent infection of L cells with the virus of lymphocytic choriomeningitis. Journal of General Virology 5:63
    [Google Scholar]
  10. Magnus P. von. 1954; Incomplete forms of influenza virus. Advances in Vims Research 2:59
    [Google Scholar]
  11. Pfau C. J. 1965; Biophysical and biochemical characterization of lymphocytic choriomeningitis virus. 2. Partial purification by differential centrifugation and fluorocarbon techniques. Acta pathologica et microbiologica scandinavica 63:198
    [Google Scholar]
  12. Pfau C. J., Camyre K. P. 1967; Biophysical and biochemical characterization of lymphocytic choriomeningitis virus. 3. Thermal and ultrasonic sensitivity. Archiv für die gesamte Virusforschung 20:430
    [Google Scholar]
  13. Pfau C. J., Camyre K. P. 1968; Inhibition of lymphocytic choriomeningitis virus multiplication by 2-(alpha-hydroxybenzyl) benzimidazole. Virology 35:375
    [Google Scholar]
  14. Pulkkinen A. J., Pfau C. J. 1970; Plaque size heterogeneity: a genetic trait of lymphocytic choriomeningitis virus. Applied Microbiology 20:123
    [Google Scholar]
  15. Rowe W. P., Murphy F. P., Berfold G. H., Casals J., Dalton A. J., Johnson K. M., Lehmann-Grube F., Mims C. A., Traub E., Webb P. A. 1970a; Arenoviruses: proposed name for a newly defined virus group. Journal of Virology 5:651
    [Google Scholar]
  16. Rowe W. P., Pugh W. E., Webb P. A., Peters C. J. 1970b; Serological relationship of the Tacaribe complex of viruses to lymphocytic choriomeningitis. Journal of Virology 5:289
    [Google Scholar]
  17. Staneck L. D., Welsh R. M., Pfau C. J. 1971; Biological activity of lymphocytic choriomeningitis virus defective particles. Bacteriological Proceedings p 206
    [Google Scholar]
  18. Sedwick W. D., Wiktor T. J. 1967; Reproducible plaquing system for rabies, lymphocytic choriomeningitis, and other RNA viruses in BHK 21/13 S agarose suspensions. Journal of Virology 1:1224
    [Google Scholar]
  19. Traub E., Resting F. 1963; Experiments on heterologous and homologous interference in LCM-infected cultures of murine lymph node cells. Archiv für die gesamte Virusforschung 14:55
    [Google Scholar]
  20. Trowbridge R. S. 1970 Ph.D. thesis University of Massachusetts; Amherst, Massachusetts:
  21. Trowbridge R. S., Pfau C. J. 1970; Persistent infection of cultured BHK 21/13 S cells by lymphocytic choriomeningitis virus. Bacteriological Proceedings p 199
    [Google Scholar]
  22. Veltri R. W., Kirk B. E. 1967; Interference between lymphocytic choriomeningitis and polio virus in tissue culture. Bacteriological Proceedings p 172
    [Google Scholar]
  23. Volkert M., Larsen J. H., Pfau C. J. 1964; Studies on immunological tolerance to LCM virus. IV. The question of immunity in adoptively immunized virus carriers. Acta pathologica et microbiologica scandinavica 61:268
    [Google Scholar]
  24. Wagner R. R., Snyder R. M. 1962; Viral interference induced in mice by acute or persistent infection with the virus of LCM. Nature, London 196:393
    [Google Scholar]
  25. Welsh R. M. 1971; Lymphocytic choriomeningitis virus defective interfering particles. Ph.D. thesis University of Massachusetts; Amherst, Massachusetts:
    [Google Scholar]
  26. Welsh R. M., Pfau C. J. 1970; Plaque assay demonstrating lymphocytic choriomeningitis virus interference. Bacteriological Proceedings p 160
    [Google Scholar]
  27. Welsh R. M., Trowbridge R. S., Kowalski J. B., O’connell C. M., Pfau C. J. 1971; Amantadine hydrochloride inhibition of early and late stages of lymphocytic choriomeningitis virus-cell interactions. Virology45–679
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-14-2-177
Loading
/content/journal/jgv/10.1099/0022-1317-14-2-177
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error