1887

Abstract

SUMMARY

Two electrophoretically distinct classes of virus particles, designated fast (AfV-F) and slow (AfV-S) according to their relative electrophoretic mobilities, were isolated from the mycelium of , strain 41871. They were almost completely separated by dialysis against 0.03-phosphate buffer, pH 7.6, in which F particles remained in suspension, while S particles precipitated, and were further purified by caesium chloride density gradient centrifugation. Electron microscopy showed that both classes were composed of isometric particles of similar diameter.

RNA, prepared from both AfV-F and AfV-S, was double-stranded. In polyacrylamide gel electrophoresis AfV-F RNA was resolved into four main components with molecular weights of 2.31, 1.87, 1.70 and 1.44 × 10, while AfV-S RNA gave two components with molecular weights of 2.76 and 2.24 × 10.

Both AfV-F and AfV-S could be separated by centrifugation in caesium chloride gradients into a number of fractions containing particles with different buoyant densities. Electrophoretic analysis of the RNA prepared from the virus fractions indicated that the multiple RNA components are not fragments released from a single virus particle, but are separately encapsidated in different particles.

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/content/journal/jgv/10.1099/0022-1317-14-2-165
1972-02-01
2024-04-25
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References

  1. Banks T., Buck K. W., Chain E. B., Darbyshire J. E., HimmelWert F. 1969; Petlicillium CyaWO-fulvum virus and interferon stimulation. Nature, London 223:155
    [Google Scholar]
  2. Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., HimmelWeit F., Ratti G., Sharpe T. J., Plan-Terose D. N. 1970; Antiviral activity of double-stranded RNA derived from a virus isolated from Aspergillus foetidus. Nature, London 227:505
    [Google Scholar]
  3. Buck K. W., Chain E. B., Himmel Weit F. 1971; Comparison of interferon induction in mice by purified Penicillium chrysogenum virus and derived double-standed RNA. Journal of General Virology 12:131
    [Google Scholar]
  4. Buck K. W., Kempson-Jones G. F. 1970; Three types of virus particle in Penicillium stoloniferum. Nature, London 225:945
    [Google Scholar]
  5. Fuji-Kawata I., Miura K., Fuke M. 1970; Segments of genome of viruses containing double-stranded ribonucleic acid. Journal of Molecular Biology 51:247
    [Google Scholar]
  6. Hanlon S., Lamer K., Lauterbach G., Johnson R., Schachman H. K. 1962; Ultracentrifuge studies with absorption optics. An automatic photoelectric scanning absorption system. Archives of Biochemistry and Biophysics 99:157
    [Google Scholar]
  7. Ifft J. B., Voet D. H., Vinograd J. 1961; The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. Journal of Physical Chemistry 65:1138
    [Google Scholar]
  8. Lhoas P. 1970; Use of heterokaryosis to infect virus free strains of Aspergillus niger. Aspergillus News Letter No. 11 8
    [Google Scholar]
  9. Loening U. E. 1967; The fractionation of high molecular weight ribonucleic acid by polyacrylamide gel electrophoresis. Biochemical Journal 102:251
    [Google Scholar]
  10. Meselson M., Stahl F. W., Vinograd J. 1957; Equilibrium sedimentation of macromolecules in density gradients. Proceedings of the National Academy of Sciences of the United States of America 43:581
    [Google Scholar]
  11. Nonoyama M., Watanabe Y., Graham A. F. 1970; Defective virions of reovirus. Journal of Virology 6:226
    [Google Scholar]
  12. Richards E. G., Coll J. A., Gratzer W. B. 1965; Disc electrophoresis of ribonucleic acid in polyacrylamide gels. Analytical Biochemistry 12:452
    [Google Scholar]
  13. Schumaker V. N., Chachman H. K. 1957; Ultracentrifugal analysis of dilute solutions. Biochimica et biophysica acta 23:628
    [Google Scholar]
  14. Shatkin A. J., Sipe J. D., Loh P. 1968; Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. Journal of Virology 2:986
    [Google Scholar]
  15. Smith L. 1968 Chromatographic and Electrophoretic Techniques Vol. 11, p 382 London: Heinemann;
    [Google Scholar]
  16. Szybalski W. 1968; Use of caesium sulphate for equilibrium density gradient centrifugation. In Methods in Enzymology vol. XIIB, p 330 Ed. by Grossman L., Moldare K. New York: Academic Press Inc;
    [Google Scholar]
  17. Wood H. A., Bozarth R. F., Mislivec P. B. 1971; Virus like particles associated with an isolate of Penicillium brevicompactum. Virology 44:592
    [Google Scholar]
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