1887

Abstract

SUMMARY

The strain of rabies virus was grown in human diploid WI-38 cells and labelled with the following radioactive substances: [C]- or [H]--glucosamine, [C]-uridine, [C]-stearic acid, [H]-choline and a mixture of [C]-amino acids. It was then purified and treated with 0.1% tri(-butyl)phosphate (TNBP) in the presence of 0.1% Tween 80. All the stearic acid and choline and about 82% and 40%, respectively, of the -glucosamine and amino acid (but none of the uridine) label was released from the virus. It was concluded that TNBP dissociates the lipids, most of the glycoproteins, and small quantities of non-glycosylated proteins from the virus leaving behind particles containing all of the virus RNA. These particles sedimented in sucrose gradients at the same rate as did intact virus; they had a higher buoyant density in CsCl gradients (1.304 compared with 1.244 g./cm.) and they resembled intact virus in overall size and shape but lacked most of the surface projections. By polyacrylamide gel electrophoresis the following polypeptide species were identified in intact particles (tentative molecular weights are given in parentheses): glycoproteins (GP1 (78,000) and GP2 (65,000); nucleocapsid proteins NP1 (58,000) and NP2 (47,000) and two additional proteins MP (35,000) and CP (22,000). Treatment with TNBP released nearly all GP1 and GP2 and small quantities of MP. Particles obtained by treatment of the virus with the proteolytic enzyme bromelain also lacked the surface projections (and the glycoproteins GP1 and GP2) but retained the lipids.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-14-1-33
1972-01-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/14/1/JV0140010033.html?itemId=/content/journal/jgv/10.1099/0022-1317-14-1-33&mimeType=html&fmt=ahah

References

  1. Cartwright B., Smale C. J., Brown F. 1969; Surface structure of vesicular stomatitis virus. Journal of General Virology 5:1
    [Google Scholar]
  2. Cartwright B., Smale C. J., Brown F. 1970a; Dissection of vesicular stomatitis virus into the infective ribonucleoprotein and immunizing components. Journal of General Virology 7:19
    [Google Scholar]
  3. Cartwright B., Smale C. J., Brown F. 1970b; Structural and biological relations in vesicular stomatitis virus. In The Biology of Large RNA Viruses pp 115–132 Ed. by Barry R. D., Mahy W. J. New York: Academic Press;
    [Google Scholar]
  4. Chen C., Compans R. W., Choppin P. W. 1971; Parainfluenza virus surface projections: glycoproteins with haemagglutinin and neuraminidase activities. Journal of General Virology 11:53
    [Google Scholar]
  5. Compans R. W. 1971; Location of the glycoprotein in the membrane of Sindbis Virus. Nature, London 229:114
    [Google Scholar]
  6. Compans R. W., Klenk H.-D., Caliguiri L. A., Choppin P. W. 1970; Influenza virus proteins. 1. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology 42:880
    [Google Scholar]
  7. Crick J., Brown F. 1970; Small immunizing subunits in rabies virus. In The Biology of Large RNA Viruses pp 133–140 Ed. by Barry R. D., Mahy B. W. J. New York: Academic Press;
    [Google Scholar]
  8. Etchison J., Doyle M., Penhoet E., Holland J. 1971; Synthesis and cleavage of influenza virus proteins. Journal of Virology 7:155
    [Google Scholar]
  9. Grimes W. J., Burge B. W. 1971; Modification of Sindbis virus glycoprotein by host-specified glycosyl transferases. Journal of Virology 7:309
    [Google Scholar]
  10. Gyorgyi E., Sokol F. 1971; The glycoprotein of rabies virus. Bacteriological Proceedings p 216 V 289
    [Google Scholar]
  11. Haslam E. A., Hampson A. W., Egan J. A., White D. O. 1970; The polypeptides of influenza virus. II,. Interpretation of polyacrylamide gel electrophoresis patterns. Virology 42:555
    [Google Scholar]
  12. Maizel J. V. JUN., White D. O., Scharff M. D. 1968; The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology 36:115
    [Google Scholar]
  13. Neurath A. R. 1966; ‘Soluble’ antigens from cells infected with rabies virus. Nature, London 212:857
    [Google Scholar]
  14. Neurath A. R., Rubin B. A. 1971; Viral structural components as immunogens of prophylactic value. Monographs in Virology vol 4 Basel: Karger;
    [Google Scholar]
  15. Neurath A. R., Rubin B. A., Sillaman J., Tint H. 1971; The effect of nonaqueous solvents on the quaternary structure of viruses: a procedure for the simultaneous concentration, purification and disruption of influenza viruses. Microbios (in the Press)
    [Google Scholar]
  16. Neurath A. R., Wiktor T. J., Koprowski H. 1966; Density gradient centrifugation studies on rabies virus. Journal of Bacteriology 92:102
    [Google Scholar]
  17. Printz P., Wagner R. R. 1971; Temperature-sensitive mutants of vesicular stomatitis virus: synthesis of virus-specific proteins. Journal of Virology 7:651
    [Google Scholar]
  18. Schlumberger H. D., Wiktor T. J., Koprowski H. 1970; Antigenic and immunologic properties of components contained in rabies virus-infected tissue culture fluids. Journal of Immunology 105:291
    [Google Scholar]
  19. Schulze L. T. 1970; The structure of influenza virus. 1. The polypeptides of the virion. Virology 42:890
    [Google Scholar]
  20. Shapiro A. L., Vinuela E., Maizel J. V. Jun. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815
    [Google Scholar]
  21. Sokol F., Kuwert E., Wiktor T. J., Hummeler K., Koprowski H. 1968; Purification of rabies virus grown in tissue culture. Journal of Virology 2:836
    [Google Scholar]
  22. Sokol F., Schlumberger H. D., Wiktor T. J., Koprowski H. 1969; Biochemical and biophysical studies on the nucleocapsid and on the RNA of rabies virus. Virology 38:651
    [Google Scholar]
  23. Sokol F., Stancek D., Koprowski H. 1971; Structural proteins of rabies virus. Journal of Virology 7:241
    [Google Scholar]
  24. Turner G. S., Kaplan C. 1967; Some properties of fixed rabies virus. Journal of General Virology 1:537
    [Google Scholar]
  25. Vinogard J., Hearst J. E. 1962; Equilibrium sedimentation of macromolecules and viruses in a density gradient. Progress in the Chemistry of Organic Natural Products 20:372
    [Google Scholar]
  26. Wiktor T. J., Fernandes M. V., Koprowski H. 1964; Cultivation of rabies virus in human diploid cell strain WI-38. Journal of Immunology 93:353
    [Google Scholar]
  27. Wiktor T. J., Sokol F., Kuwert E., Koprowski H. 1969; Immunogenicity of concentrated and purified rabies vaccine of tissue culture origin. Proceedings of the Society for Experimental Biology and Medicine, N. Y 131:799
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-14-1-33
Loading
/content/journal/jgv/10.1099/0022-1317-14-1-33
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error