Varicella-zoster (V-Z) virus was first isolated by Weller (1953) and has been classified as a group B cell-associated herpesvirus of man (Melnick 1964). The cell-associated nature of the virus has made it difficult to obtain infectious virus from tissue culture and to prepare large quantities of virus particles for the characterization of the virion and its DNA. In this report we describe the successful purification of V-Z virus and define some of the properties of its DNA.

Two strains of V-Z virus were used in this study. Strain 182 was isolated from a child who had expired with generalized varicella (Benyesh-Melnick, Rosenberg & Watson, 1964), and strain was obtained from a patient with generalized herpes zoster infection (Rapp & Benyesh-Melnick, 1963). Both strains were propagated in human embryonic lung (HEL) fibroblasts as described previously (Rapp & Benyesh-Melnick, 1963). Intact infected cells were mixed with freshly trypsinized normal fibroblasts at a ratio of 1:10.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error