1887

Abstract

Studies of genetic recombination have been of considerable value in elucidating the structure and function of bacterial viruses and have also been of value for certain animal viruses (e.g. see Fenner, 1970). Pringle (1970) has reported recombination between mutants of vesicular stomatitis virus (VSV) when mutants from different complementation groups were used in mixed infections. We have looked for recombination in our collection of VSV mutants (Holloway, Wong & Cormack, 1970), also, and have obtained the results presented in this paper.

L-cell and virus stocks were maintained and assayed using the methods previously described (Holloway 1970). For the present experiments, cell monolayers were prepared by seeding each of a number of 2 oz. Brockway bottles with 2.5 × 10 cells. For mixed infection 2.5 × 10 p.f.u. of each mutant were added in a total volume of 0.5 ml, giving a total m.o.i. of 20.

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/content/journal/jgv/10.1099/0022-1317-13-3-477
1971-12-01
2019-10-21
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