RT Journal Article SR Electronic(1) A1 Bell, D. A1 Wilkie, N. M. A1 Subak-Sharpe, J. H.YR 1971 T1 Studies on Arginyl Transfer Ribonucleic Acid in Herpes Virus Infected Baby Hamster Kidney Cells JF Journal of General Virology, VO 13 IS 3 SP 463 OP 475 DO https://doi.org/10.1099/0022-1317-13-3-463 PB Microbiology Society, SN 1465-2099, AB SUMMARY Previous work, (Subak-Sharpe & Hay, 1965, Subak-Sharpe, Shepherd & Hay, 1966), depending on the hybridization of [32P]4s RNA from herpes virus infected cells to herpes DNA and analysis of the aminoacyloligonucleotide fragments produced after RNase T 1 digestion of [14C]- and [3H]-arginyl tRNA, suggested the presence, in infected cells of a herpes-specified arginyl tRNA. The present study indicates that these results were due to the presence of (a) radioactive RNA other than tRNA in the [32P] 4sRNA, and (b) radioactive contaminants in the [3H]-arginine used to aminoacylate the tRNA. Further purification of the aminoacyl tRNA removes these [3H]-arginine contaminants. Protection of the aminoacyl tRNA ester bond by N-acetylation improved the sensitivity of the aminoacyloligonucleotide analyses and permitted tRNA-specific molecular hybridization. Using these techniques, no herpes-specified arginyl tRNA was detected in cells 7 to 9 hr post infection., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-13-3-463