1887

Abstract

SUMMARY

Previous work, (Subak-Sharpe & Hay, 1965, Subak-Sharpe, Shepherd & Hay, 1966), depending on the hybridization of [P]4s RNA from herpes virus infected cells to herpes DNA and analysis of the aminoacyloligonucleotide fragments produced after RNase T 1 digestion of [C]- and [H]-arginyl tRNA, suggested the presence, in infected cells of a herpes-specified arginyl tRNA. The present study indicates that these results were due to the presence of () radioactive RNA other than tRNA in the [P] 4sRNA, and () radioactive contaminants in the [H]-arginine used to aminoacylate the tRNA. Further purification of the aminoacyl tRNA removes these [H]-arginine contaminants. Protection of the aminoacyl tRNA ester bond by -acetylation improved the sensitivity of the aminoacyloligonucleotide analyses and permitted tRNA-specific molecular hybridization. Using these techniques, no herpes-specified arginyl tRNA was detected in cells 7 to 9 hr post infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-13-3-463
1971-12-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/13/3/JV0130030463.html?itemId=/content/journal/jgv/10.1099/0022-1317-13-3-463&mimeType=html&fmt=ahah

References

  1. Bollum F. J. 1959; Thermal conversion of nonpriming deoxyribonucleic acid to primer. Journal of Biological Chemistry 234:2733
    [Google Scholar]
  2. Daniel V., Sarid S., Littauer U. Z. 1970; Bacteriophage induced transfer RNA in Escherichia coli . Science, New York 167:1682
    [Google Scholar]
  3. De Groot N., Lapidot Y., Panet A., Wolman Y. 1966; The synthesis of JV-acetylphenylalanyl-sRNA. Biochemical and Biophysical Research Communications 25:17
    [Google Scholar]
  4. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilised on a membrane. Journal of Molecular Biology 12:829
    [Google Scholar]
  5. House W., Wildy P. 1965; Large scale production of tissue cells and virus. Laboratory Practise 14:594
    [Google Scholar]
  6. Hsu W. T., Foft J. W., Weiss S. B. 1967; Effect of bacteriophage infection on the sulfur labelling of sRNA. Proceedings of the National Academy of Sciences of the United States of America 58:2028
    [Google Scholar]
  7. Kozinski A. W., Szybalski W. 1959; Dispersive transfer of the parental DNA molecule to the progeny of Phage ϕ × 174. Virology 9:260
    [Google Scholar]
  8. Landy A., Abelson J., Goodman N. M., Smith J. D. 1967; Specific hybridisation of tyrosine tRNAs with DNA from a transducing bacteriophage ϕ 80 carrying the amber suppressor gene su III . Journal of Molecular Biology 29:457
    [Google Scholar]
  9. Lapidot Y., De Groot N., Fry-Shafir I. 1967; Modified Aminoacyl-tRNA II. A general method for the preparation of Acyl-aminoacyl-tRNA. Biochimica et biophysica acta 145:292
    [Google Scholar]
  10. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones-an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  11. Morris V. L., Wagner E. K., Roizman B. 1969; Arginyl transfer RNA populations in human (HEp-2) cells infected with herpes simplex virus. Bacteriological Proceedings170
    [Google Scholar]
  12. Morris V. L., Wagner E. K., Roizman B. 1970; RNA synthesis in cells infected with herpes simplex virus III. Absence of virus specified arginyl-and seryl-tRNA in infected Hep-2 cells. Journal of Molecular Biology 52:247
    [Google Scholar]
  13. Raška K., Frohwirth D. H., Schlesinger R. W. 1970; Transfer ribonucleic acid in KB cells infected with adenovirus type 2. Journal of Virology 5:464
    [Google Scholar]
  14. Ritchie D. A., Malcolm F. E. 1970; Heat stable and density mutants of phages, T1 T3 and T7 . Journal of General Virology 9:35
    [Google Scholar]
  15. Rosenbloom J., Cox E. C. 1966; Sedimentation coefficient of T-Even Bacteriophage DNA. Biopolymers 4:747
    [Google Scholar]
  16. Russell W. C., Crawford L. V. 1963; Some characteristics of the deoxyribonucleic acid from herpes simplex virus. Virology 21:353
    [Google Scholar]
  17. Scherberg N. H., Guha A., Hsu W.-T., Weiss S. B. 1970; Evidence for the early synthesis of T4 bacteriophage-coded transfer RNA. Biochemical and Biophysical Research Communications 40:919
    [Google Scholar]
  18. Subak-Sharpe H., Hay J. 1965; An animal virus with DNA of high guanine + cytosine content which codes for sRNA. Journal of Molecular Biology 12:924
    [Google Scholar]
  19. Subak-Sharpe H., Shepherd W., Hay J. 1966; Studies on sRNA coded by herpes virus. Cold Spring Harbour Symposium on Quantitative Biology 31:583
    [Google Scholar]
  20. Thomas C. A., Abelson J. 1966; The isolation and characterization of DNA from bacteriophage. In Procedures in Nucleic Acid Research p 553 Ed. Cantoni G. L., Davies D. R. New York: Harper and Row;
    [Google Scholar]
  21. Waters L. C., Novelli G. D. 1967; A new change in leucine transfer RNA observed in Escherichia coli infected with bacteriophage T2 . Proceedings of the National Academy of Sciences of the United States of America 57:979
    [Google Scholar]
  22. Weiss S. B., Hsu W.-T., Foft J. W., Scherberg N. H. 1968; Transfer RNA coded by the T4 bacteriophage genome. Proceedings of the National Academy of Sciences of the United States of America 61:114
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-13-3-463
Loading
/content/journal/jgv/10.1099/0022-1317-13-3-463
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error