1887

Abstract

SUMMARY

Semliki Forest virus was thermally inactivated between 20° and 50° by two processes, one of which predominated at temperatures below 41° and the other at higher temperatures. At pH 6.5, the rates of inactivation were greater than at pH 7.5 but the nature of the reactions was unchanged. The stability of the virus in phosphate buffer solutions was greatly reduced at lower concentrations of protein in the suspending medium. The rate of inactivation was reduced in the dark. At higher temperatures, a change occurred in the surface properties of the virus that did not, of itself, cause loss of infectivity.

It is suggested that at the higher temperatures the inactivation was a consequence of a structural breakdown of a surface unit in the virus; at lower temperatures a more subtle change in the substructure was responsible for inactivation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-13-3-385
1971-12-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/13/3/JV0130030385.html?itemId=/content/journal/jgv/10.1099/0022-1317-13-3-385&mimeType=html&fmt=ahah

References

  1. Bachrach H. L., Breese S. S., Callis J. J., Hess W. R., Patty R. E. 1957; Inactivation of foot-and-mouth disease virus by pH and temperature changes and by formaldehyde. Proceedings of the Society for Experimental Biology and Medicine 95:147
    [Google Scholar]
  2. Bradish C. J., Allner K., Maber H. B. 1971; The virulence of original and derived strains of Semliki Forest virus for mice, guinea-pigs and rabbits. Journal of General Virology 12:141
    [Google Scholar]
  3. Bradish C. J., Farley J. O., Ferrier H. E. N. 1962; Studies on the nature of the neutralisation reaction and the competition for neutralising antibody between components of the virus system of foot-and-mouth disease virus. Virology 18:378
    [Google Scholar]
  4. Brownhill T. J., Tones A. S., Stacey M. 1959; The inactivation of ribonuclease during the isolation of ribonucleic acids and ribonuclear proteins. Biochemical Journal 73:434
    [Google Scholar]
  5. Dimmock N. 1967; Differences between thermal inactivation of picornaviruses at high and low temperatures. Virology 31:338
    [Google Scholar]
  6. Eigner J., Boedtker H., Michaels G. 1961; The thermal degradation of nucleic acids. Biochimica et biophysica acta 51:165
    [Google Scholar]
  7. Ginoza W. 1958; Kinetics of heat inactivation of ribonucleic acid and tobacco mosaic virus. Nature, London 181:958
    [Google Scholar]
  8. Kaplan C. 1958; Heat inactivation of vaccinia virus. Journal of General Microbiology 18:58
    [Google Scholar]
  9. March R. W., Hetrick F. M. 1967; Studies on Guaroa virus: Virus characteristics. American Journal of Tropical Medicine and Hygiene 16:191
    [Google Scholar]
  10. Osterreith P. M. 1964; Heat inactivation of Semliki Forest virus. Acta Virologica 8:476
    [Google Scholar]
  11. Plummer G., Lewis B. 1965; Thermoinactivation of herpes simplex virus and cytomegalovirus. Journal of Bacteriology 89:671
    [Google Scholar]
  12. Simpson R. W., Hauser R. E. 1968; Effect of heat on virions of wild-type Sindbis virus and a thermal stable mutant. Virology 34:361
    [Google Scholar]
  13. Singer B., Fraenkel-Conrat H. 1963; The nature of breaks occurring in tobacco mosaic virus ribonucleic acid under various conditions. Biochimica et biophysica acta 76:143
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-13-3-385
Loading
/content/journal/jgv/10.1099/0022-1317-13-3-385
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error