Polyoma virus can follow two distinct paths according to the nature of the cells supporting its development: a lytic or productive cycle in permissive cells and a transforming cycle either abortive or complete. Virus infection always induces the production of the same new antigen which, according to Huebner's terminology (1967), is the T antigen in the lytic cycle and the tumour antigen in the transforming cycles. In the lytic cycle, with a high enough infection multiplicity, the T antigen appears early and disappears when the structural antigens of the virus are synthesized (Takemoto, Malmgren & Habel, 1966). We studied the kinetics of synthesis and the renewal rate of T antigen in mouse embryo fibroblasts infected with polyoma virus (lytic cycle) after labelling the T antigen with [H]-leucine or with a mixture of [C]-amino acids. After fractionation of the nuclei, the T antigen in the nuclear proteins was titrated by a radio-immunological method.


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