1887

Abstract

SUMMARY

Feline leukaemia virus and the feline leukaemia virus pseudotype of murine sarcoma (MSV(FeLV)) readily propagate in cat embryo cells. Feline leukaemia virus will rapidly attain high titres even if minimal virus inocula are used initially, and undiminished virus production will be maintained in the chronically infected cells. Feline leukaemia virus, which was previously quantitated as helper activity for defective MSV (FeLV), has now been related to the amount of autonomously replicating feline leukaemia virus. Rapid growth of MSV (FeLV) to high titre is obtained only if each sarcoma infected cell also becomes the recipient of a replicating unit of feline leukaemia virus. Conditions approximating one-step growth curves for both feline leukaemia virus alone and MSV (FeLV)–feline leukaemia virus complex can be achieved and show that first cycles of growth are completed within about 40 and 30 hr, respectively. The rate of growth of the sarcoma-leukaemia virus complex is somewhat faster than that of leukaemia virus by itself, which can result in ratios of replicating leukaemia to sarcoma viruses of less than unity. Inhibition of DNA synthesis during the first 12 hr after infection with either virus prevents virus replication. The requirement for DNA synthesis is not seen beyond the first 12 hr after infection. No stimulation of DNA synthesis is discernible after either feline leukaemia virus or MSV (FeLV) inoculation at any time after infection.

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1971-11-01
2022-01-21
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References

  1. Bassin R. H., Simons A. H., Chesterman F. C., Harvey J. J. 1968; Murine sarcoma virus (Harvey) characteristics of focus formation in mouse embryo cell cultures, and virus production by hamster tumor cells. International Journal of Cancer 3:265
    [Google Scholar]
  2. Chapman A., Fischinger P. J., O’connor T. E. 1970; Infection and transformation of dog cells with a modified sarcoma virus. Journal of the National Cancer Institute 45:1047
    [Google Scholar]
  3. Fischinger P. J., O’connor T. E. 1968; Tissue culture assay of helper activity of murine leukemia virus for murine sarcoma virus. Journal of the National Cancer Institute 40:1199
    [Google Scholar]
  4. Fischinger P. J., O’connor T. E. 1969a; Viral infection across species barriers: alteration of murine sarcoma virus for growth in cat cells. Science, New York 165:714
    [Google Scholar]
  5. Fischinger P. J., O’connor T. E. 1969b; Radiation leukemia virus: quantitative tissue culture assay. Science, New York 165:306
    [Google Scholar]
  6. Fischinger P. J., O’connor T. E. 1970; Productive infection and morphologic alteration of human cells by a modified sarcoma virus. Journal of the National Cancer Institute 44:429
    [Google Scholar]
  7. Hahn G. M., Decleve A., Lieberman M., Kaplan H. S. 1970; Focus formation by a murine sarcoma-leukemia complex. I. Theoretical analysis. Journal of Virology 5:432
    [Google Scholar]
  8. Hartley J., Rowe W. 1966; Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proceedings of the National Academy of Sciences of the United States of America 55:780
    [Google Scholar]
  9. Hirschman S. Z., Fischinger P. J., Zaccari J. J., O’connor T. E. 1969a; Effect of cytosine arabinoside on the replication of Moloney sarcoma virus in 3 T3 cell cultures. Journal of the National Cancer Institute 42:399
    [Google Scholar]
  10. Hirschman S. Z., Fischinger P. J., O’connor T. E. 1969b; The inhibition by cytosine arabinoside of the replication of murine leukemia and sarcoma viruses in mouse embryo cultures. International Journal of Cancer 4:671
    [Google Scholar]
  11. Hirschman S. Z., Fischinger P. J., O’connor T. E. 1970; Effect of infection with Moloney sarcoma and leukemia viruses on nucleic acid synthesis in mouse cell cultures. Journal of the National Cancer Institute 44:107
    [Google Scholar]
  12. Jarrett O., Laird H. M., Hay D., Crighton G. W. 1968; Replication of cat leukeamia virus in cell cultures. Nature, London 219:521
    [Google Scholar]
  13. Jarrett O., Laird H. M., Hay D. 1969; Growth of feline leukemia virus in human, canine and porcine cells. Comparative Leukemia Research, 1969. Bibl. Haemat., no. 36 p 387 Ed. by Dutcher R. M. Basel, Miinchen, Paris, New York: Karger (1970);
    [Google Scholar]
  14. Nakata Y., Bader J. 1968; Transformation of murine sarcoma virus: fixation (desoxyribonucleic acid synthesis) and development. Journal of Virology 2:1255
    [Google Scholar]
  15. O’connor T. E., Fischinger P. J. 1968; Titration patterns of a murine sarcoma-, leukemia virus complex: evidence for existence of competent sarcoma virions. Science, New York 159:325
    [Google Scholar]
  16. O’connor T. E., Fischinger P. J. 1969a; Physical properties of competent and defective states of murine sarcoma (Moloney) virus. Journal of the National Cancer Institute 43:487
    [Google Scholar]
  17. O’connor T. E., Fischinger P. J. 1969b; Physical alterations of a murine leukemia virus complex in mammalian cell cultures. Comparative Leukemia Research, 1969. Bibl. Haemat., no. 36 p 250 Ed. by Dutcher R. M. Basel, Miinchen, Paris, New York: Karger (1970);
    [Google Scholar]
  18. Pring M. 1967; The influence of dilution on the probability of multiple infection of cells by virus particles of different types. Proceedings of the National Academy of Sciences of the United States of America 58:607
    [Google Scholar]
  19. Purifoy D. J. M., Purifoy J. A., Sagik B. P. 1968; A mathematical analysis of concomitant virus replication and heat inactivation. Journal of Virology 2:275
    [Google Scholar]
  20. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent end points. American Journal of Hygiene 27:493
    [Google Scholar]
  21. Rickard C. G., Post J. E., Noronha F., Barr L. M. 1969; A transmissible virus-induced lymphcytic leukemia of the cat. Journal of the National Cancer Institute 42:987
    [Google Scholar]
  22. Sarma P. S., Log T., Huebner R. S. 1970; Trans-species rescue of defective genomes of murine sarcoma virus from hamster tumor cells with helper feline leukemia virus. Proceedings of the National Academy of Sciences of the United States of America 65:81
    [Google Scholar]
  23. Schmidt G., Thannhauser S. J. 1945; A method for determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues. Journal of Biological Chemistry 161:83
    [Google Scholar]
  24. Sibal L. R., Fink M. A., Plata E. J., Kohler B. E., Noronha F., Lee K. M. 1970; Methods for the detection of viral antigen and antibody to a leukemia virus (a preliminary report). Journal of the National Cancer Institute 45:607
    [Google Scholar]
  25. Spiegelman S., Burny A., Das A., Keydar J., Schlom J., Travnicek M., Watson K. 1970; Physical and chemical characterization of the products of RNA-directed DNA polymerases in oncogenic RNA viruses. Nature, London 227:563
    [Google Scholar]
  26. Temin H. M. 1967; Studies on carcinogenesis by avian sarcoma viruses. V. Requirement for new DNA synthesis and for cell division. Journal of Cellular Physiology 69:53
    [Google Scholar]
  27. Temin H. M., Mizutani S. 1970; RNA dependent DNA polymerase in virions of Rous sarcoma virus. Nature, London 2261211
    [Google Scholar]
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