Electron Microscopic Study of Development of Vesicular Stomatitis Virus Using Ferritin-labelled Antibodies

Significant virus autointerference was not observed in serial undiluted passages of vesicular stomatitis virus in Madin-Darby canine kidney cells. The ultra-structural features of vesicular stomatitis virus development into Madin-Darby canine kidney cells were studied by staining frozen-thawed cells with ferritin-conjugated antibody. The granular and fibrillar antigen could be readily identified in the cytoplasm within 4 hr after infection. Its appearance was followed closely by the differentiation of virus filaments at the cell surface. The virus antigen could be recognized in close proximity to virus particles; this material may represent virus nucleoprotein incorporated into budding virus particles.