1887

Abstract

SUMMARY

The virus RNAs and proteins synthesized in chick embryo cells infected with different strains of Newcastle disease virus have been characterized by polyacrylamide gel electrophoresis. By comparison of the rates of electrophoretic mobility of virus RNA with those of RNA molecules of known molecular weight, the molecular weights were estimated to be 4.8 × 10 for the virus particle RNA and 2.5 × 10, 1.1 × 10 and 0.4 to 0.7 × 10 for the RNA molecules synthesized in infected cells, the latter being heterogeneous. Synthesis of a number of virus particle proteins was readily detected.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-13-1-111
1971-10-01
2024-06-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/13/1/JV0130010111.html?itemId=/content/journal/jgv/10.1099/0022-1317-13-1-111&mimeType=html&fmt=ahah

References

  1. Barry R. D., Bukrinskaya A. G. 1968; The nucleic acid of Sendai virus and ribonucleic acid synthesis in cells infected by Sendai virus. Journal of General Virology 2:71
    [Google Scholar]
  2. Bishop D. H. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373
    [Google Scholar]
  3. Blair C. D., Robinson W. S. 1968; Republication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology 35:537
    [Google Scholar]
  4. Bratt M. A. 1969; RNA synthesis in chick embryo cells infected with different strains of NDV. Virology 38:485
    [Google Scholar]
  5. Bratt M. A., Robinson W. S. 1967; Ribonucleic acid synthesis in cells infected with Newcastle disease virus. Journal of Molecular Biology 23:1
    [Google Scholar]
  6. Caliguiri L. A., Klenk H. D., Choppin P. W. 1969; The proteins of the parainfluenza virus SV5. 1. Separation of virion polypeptides by polyacrylamide gel electrophoresis. Virology 39:460
    [Google Scholar]
  7. Compans R. W., Choppin P. W. 1968; The nucleic acid of the parainfluenza virus SV5. Virology 35:289
    [Google Scholar]
  8. Duesberg P. H., Robinson W. S. 1965; Isolation of the nucleic acid of Newcastle disease virus (NDV). Proceedings of the National Academy of Sciences of the United States of America 54:794
    [Google Scholar]
  9. Evans M. J., Kingsbury D. W. 1969; Separation of Newcastle disease virus proteins by polyacrylamide gel electrophoresis. Virology 37:597
    [Google Scholar]
  10. Gandhi S. S., Burke D. C. 1970; Interferon production by myxoviruses in chick embryo cells. Journal of General Virology 6:95
    [Google Scholar]
  11. Hanson R. P., Brandly C. A. 1955; Identification of vaccine strains of Newcastle disease virus. Science, New York 122:156
    [Google Scholar]
  12. Haslam E. A., Cheyne I. M., White D. O. 1969; The structural proteins of Newcastle disease virus. Virology 39:118
    [Google Scholar]
  13. Hay A. J., Skehel J. J., Burke D. C. 1968; Proteins synthesised in chick cells following infection with Semliki Forest virus. Journal of General Virology 3:175
    [Google Scholar]
  14. Huang A. S., Baltimore D., Stampfer M. 1970; Ribonucleic acid synthesis of vesiculars tomatitis virus. III. Multiple complementary messenger RNA molecules. Virology 42:946
    [Google Scholar]
  15. Joss A. Gandhi S. S., Hay A. J., Burke D. C. 1969; Ribonucleic acid and protein synthesis in chick embryo cells infected with fowl plague virus. Journal of Virology 4:816
    [Google Scholar]
  16. Kingsbury D. W. 1967; Newcastle disease virus complementary RNA: its relationship to the viral genome and its accumulation in the presence or absence of actinomycin D. Virology 33:227
    [Google Scholar]
  17. Kingsbury D. W. 1970; Republication and functions of myxovirus ribonucleic acids. Progress in Medical Virology 12:49
    [Google Scholar]
  18. Kohn A., Fuchs P. 1969; Cell fusion by various strains of Newcastle Disease virus and their virulence. Journal of Virology 3:539
    [Google Scholar]
  19. Loening U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251
    [Google Scholar]
  20. Lomniczi B. 1970; Systemic induction of interferon in chicks with various NDV strains. I. Relationship between virulence of the virus and the mechanism of interferonformation. Archiv für die gesamte Virusforschung 30:159
    [Google Scholar]
  21. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses: a comparison of Simianvirus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 7:47
    [Google Scholar]
  22. Nakijima H., Obara J. 1967; Physiochemical studies of Newcastle disease virus. III. The content of virus nucleic acid and its sedimentation pattern. Archiv für die gesamte Virusforschung 20:287
    [Google Scholar]
  23. Peacock A. C., Dingman C. W. 1967; Resolution of multiple RNA species by polyacrylamide gel electrophoresis. Biochemistry 6:1818
    [Google Scholar]
  24. Penman S. 1966; RNA metabolism in the HeLa cell nucleus. Journal of Molecular Biology 17:117
    [Google Scholar]
  25. Penman S., Vesco C., Penman M. 1968; Localisation and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome associated messenger RNA in HeLa cells. Journal of Molecular Biology 34:49
    [Google Scholar]
  26. Petermann M. L., Pavlovec A. 1966; The subunits and structural ribonucleic acids of Jensen Sarcoma ribosomes. Biochemica et biophysica acta 114:264
    [Google Scholar]
  27. Reeve P., Alexander D. J. 1970; Plaque formation, cell fusion and haemadsorption by Newcastle disease virus. Cytobios 2:5
    [Google Scholar]
  28. Reeve P., Rosenblum M., Alexander D. J. 1970; Growth in chick chorioallantoic membranes of strains of Newcastle disease virus of differing virulence. Journal of Hygiene, Cambridge 68:61
    [Google Scholar]
  29. Reeve P., Waterson A. P. 1970; The growth cycle of avirulent strains of Newcastle disease virus. Microbios 2:5
    [Google Scholar]
  30. Schloer G. M., Hanson R. P. 1968; Relationship of plaque size and virulence for chickens of 14 representative Newcastle disease virus strains. Journal of Virology 2:40
    [Google Scholar]
  31. Spirin A. S. 1963; Some problems concerning the macromolecular structure of ribonucleic acids. In Progress in Nucleic Acid Research vol 1 p 301 Ed. by Davidson J. N, Cohn W. E. New York: Academic Press;
    [Google Scholar]
  32. Wagner E. K., Katz L., Penman S. 1967; The possibility of aggregation of ribosomal RNA during hot phenol-SDS deproteinisation. Biochemical and Biophysical Research Communications 28:152
    [Google Scholar]
  33. Walters S., Burke D. C., Skehel J. J. 1967; Interferon production and RNA inhibitors. Journal of General Virology 1:349
    [Google Scholar]
  34. Waterson A. P., Pennington T. H., Allan W. H. 1967; Virulence in Newcastle disease virus. A preliminary study. British Medical Bulletin 23(2):138
    [Google Scholar]
  35. Weinberg R. A., Penman S. 1970; Processing of 45s nucleolar RNA. Journal of Molecular Biology 47:169
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-13-1-111
Loading
/content/journal/jgv/10.1099/0022-1317-13-1-111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error