1887

Abstract

SUMMARY

Two established rat cell lines, WERC and TWERC, were studied for the presence of C-type RNA virus particles. WERC cells, spontaneously transformed , continuously released C-type RNA virus (WERC virus) by budding from the plasma membrane. Some of these virus particles (diameter about 100 nm.) showed an electron-lucent nucleoid of diameter 75 to 80 nm. and represented immature C-type virus. Others showed dense nucleoids of about 65 to 70 nm. diameter and looked like mature forms of murine leukaemia virus. Each cell showed 60 to 100 virus particles. The buoyant density of the virus particles in sucrose gradients was that expected for avian or murine RNA virus particles (1.16 g./ml.).

No virus particles were observed in the subline of cells (TWERC) originating from the malignant transformation with avian sarcoma virus (the strain of Rous sarcoma virus) of parental WERC cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-12-3-303
1971-09-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/12/3/JV0120030303.html?itemId=/content/journal/jgv/10.1099/0022-1317-12-3-303&mimeType=html&fmt=ahah

References

  1. Bernhard W. 1960; The detection and study of tumour virus with the electron microscope. Cancer Research 20:712
    [Google Scholar]
  2. De-Thé G. B., O’Connor T. E. 1966; Structure of murine leukemia virus after disruption with tween-ether and comparison with two myxoviruses. Virology 28:713
    [Google Scholar]
  3. Dougherty R. M., Di Stefano H. S. 1965; Virus particles associated with ‘non-producer’ Rous sarcoma cells. Virology 27:351
    [Google Scholar]
  4. Duesberg P. H., Robinson W. K. 1966; Nucleic acid and proteins isolated from the Rauscher leukemia virus (MLV). Proceedings of the National Academy of Sciences of the United States of America 55:219
    [Google Scholar]
  5. Earle W. R. 1943; Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. Journal of the National Cancer Institute 4:165
    [Google Scholar]
  6. Earle W. R., Nettleship A. 1943; Production of malignancy in vitro. V. Results of injections of cultures into mice. Journal of the National Cancer Institute 4:213
    [Google Scholar]
  7. Firor W. M., Gey G. O. 1945; Observations on the conversion of normal into malignant cells. Annals of Surgery 121:700
    [Google Scholar]
  8. Gey G. O. 1941; Cytological and cultural observations on transplantable rat sarcomata produced by the inoculation of altered normal cells maintained in continuous culture. Cancer Research 1:737
    [Google Scholar]
  9. Huebner R. J., Todaro G. S. 1969; Oncogenes of RNA tumor viruses as determinants of cancer. Proceedings of the National Academy of Sciences of the United States of America 64:1087
    [Google Scholar]
  10. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409
    [Google Scholar]
  11. Sanford K. K. 1965; Malignant transformation of cells in vitro . International Review of Cytology 18:249
    [Google Scholar]
  12. Sharon N., Pollard M. 1969; Spontaneous neoplastic transformation of germ-free rat embryo cell culture. Cancer Research 29:1523
    [Google Scholar]
  13. Šimkovič D., Valentová N., Thurzo V. 1967; Malignant conversion of rat cells in vitro by mixed cultivation with chicken Rous sarcoma cells into nonproducing non-virogenic tumour cells. First European Cancer Congress (June 30 to July 3) Vienna, Austria:
    [Google Scholar]
  14. Šimkovič D., Adamcová B. 1969; Some problems of detection of genome of Rous sarcoma virus in heterologous cells. Progress in Immunobiological Standardization vol 3 p. 49 Basel, New York: Karger;
    [Google Scholar]
  15. Temin H. M., Rubin H. 1958; Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture. Virology 6:669
    [Google Scholar]
  16. Veselý P., Donner L., Kučerová M. 1968; Spontaneous transformation of embryonic rat fibroblasts from an inbred lewis strain in vitro . Folia hiologica (Praha) 14:409
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-12-3-303
Loading
/content/journal/jgv/10.1099/0022-1317-12-3-303
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error