Ultrastructural Features of Canine Distemper Virus Infection in a Dog Kidney Cell Line Free

Abstract

SUMMARY

Cultures of dog kidney cells were infected with canine distemper virus at an input multiplicity of 1.9 and the subsequent events followed by electron microscopy, conventional staining methods and virus titration. Maturation and release of virus commenced within 24 hr of infection and was a slow but prolonged process, the infected cell producing small amounts of virus for at least 48 hr. After formation in cytoplasmic foci, often perinuclear, the virus nucleocapsid migrated to the cell membrane, below which it adopted a symmetrical configuration, the cell membrane at the same time acquiring a layer of fine surface projections. Maturation of virus then occurred by a protrusion and pinching off of these areas. Cytoplasmic aggregates of nucleocapsid were not detected by light microscopy. The characteristic phloxinophilic cytoplasmic inclusions, which appeared between 24 and 48 hr after infection, did not stain with acridine orange. Some consisted of a mass of amorphous electron-dense material and others of nucleocapsid-like filaments enmeshed in an electron-dense matrix but all contained a number of pockets in which membrane-bound vacuoles and tubules were prominent.

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1971-09-01
2024-03-29
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References

  1. Bittle J. L., York C. J., Newberne J. W. 1961; Adaptation and modification of a strain of canine distemper virus in tissue culture. Cornell Veterinarian 51:359
    [Google Scholar]
  2. Bussell R. H., Karzon D. T. 1962; Canine distemper virus in chick embryo cell culture. Virology 18:589
    [Google Scholar]
  3. Bussell R. H., Karzon D. T. 1965; Canine distemper virus in ferret, dog and bovine kidney cell cultures. Archiv für die gesamte Virusforschung 17:163
    [Google Scholar]
  4. Cornwell H. J. C., Campbell R. S. F., Vantsis J. T., Penny W. 1965; Studies in experimental canine distemper. I. Clinicopathological findings. Journal of Comparative Pathology 75:3
    [Google Scholar]
  5. Cornwell H. J. C., Weir A. R., Wright N. G., Thompson H. 1970; The susceptibility of a dog kidney cellline (MDCK) to canine distemper, infectious canine hepatitis and canine herpes viruses. Research in Veterinary Science 11:580
    [Google Scholar]
  6. Harrison M. J., Smith F. A., Graydon J. J. 1968; The virus of canine distemper in cell-culture. I. Adaptation of canine distemper virus to growth and serial passage in ferret kidney cell cultures and in BS-C-1 cell cultures. Journal of Comparative Pathology 78:121
    [Google Scholar]
  7. Karber G. 1931; Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Archiv für experimentelle Pathologie und Pharmakologie 162:480
    [Google Scholar]
  8. Koestner A., Long J. F. 1970; Ultrastructure of canine distemper virus in explant tissue cultures of canine cerebellum. Laboratory Investigation 23:196
    [Google Scholar]
  9. Lawn A. M. 1970; Ultrastructural features of canine distemper virus infection of the chorioallantoic membrane of the hen’s egg. Journal of General Virology 8:157
    [Google Scholar]
  10. Nakai M., Imagawa D. T. 1969; Electron microscopy of measles virus replication. Journal of Virology 3:187
    [Google Scholar]
  11. Nakai T., Shand F. L., Howatson A. F. 1969; Development of measles virus in vitro . Virology 38:50
    [Google Scholar]
  12. Raine C. S., Feldman L. A., Sheppard R. D., Bornstein M. B. 1969; Ultrastructure of measles virus in cultures of hamster cerebellum. Journal of Virology 4:169
    [Google Scholar]
  13. Rockborn G. 1958; Canine distemper virus in tissue culture. Archiv für die gesamte Virusforschung 8:485
    [Google Scholar]
  14. Shaver D. N., Bussell R. H., Barron A. L. 1964; Comparative cytopathology of canine distemper and measles viruses in ferret kidney cell-cultures. Archiv für die gesamte Virusforschung 14:487
    [Google Scholar]
  15. Shishido A., Yamanouchi K., Hikitha M., Sato T., Fukuda A., Kobune F. 1967; Development of a Cell culture system susceptible to measles, canine distemper and rinderpest viruses. Archiv für die gesamte Virusforschung 22:364
    [Google Scholar]
  16. Toyoshima K., Hata S., Takahashi M., Miki T., Okuno Y. 1960; Virological studies on measles virus. III. Morphological changes and virus growth in FL cultures. Biken’ Journal 3:241
    [Google Scholar]
  17. Vantsis J. T. 1959; Preliminary note on the propagation of canine distemper virus in different tissue-culture systems. Veterinary Record 71:99
    [Google Scholar]
  18. Yamanouchi K., Kobune F., Fukuda A., Hayami M., Shishido A. 1970; Comparative immunofluorescent studies on measles, canine distemper and rinderpest viruses. Archiv für die gesamte Virusforschung 29:90
    [Google Scholar]
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