1887

Abstract

SUMMARY

RNA from purified bovine enterovirus (serotype VG-5-27) was fractionated by sedimentation on sucrose gradients, gel filtration through Sepharose 2B and electrophoresis in acrylamide-agarose gels. Virus RNA was single stranded with a molecular weight of approximately 2.8 × 10. The RNA obtained from virus infected cells was separated into replicative intermediate and single-stranded molecules by gel filtration through Sepharose 2B. Electrophoresis of the replicative intermediate fraction on acrylamide-agarose gels gave two peaks of radioactivity, whereas after ribonuclease treatment only a single peak was present. Electrophoresis of the single-stranded molecules on acrylamide gels showed that a portion of these had slower mobilities than RNA from purified virus. This single-stranded fraction contained molecules with molecular weights in the range 5.6 to 2.8 × 10. Continuous labelling experiments showed that this size range was a function of time after infection. The existence of single strands of RNA longer than RNA found in purified virus was interpreted as supporting a cyclic model for RNA replication.

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1971-09-01
2022-09-27
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References

  1. Baltimore D. 1969; Replication of picornaviruses. In The Biochemistry of Viruses p 101 Ed. by Levy H. B. New York:: M. Dekker.;
    [Google Scholar]
  2. Bishop D. H. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373
    [Google Scholar]
  3. Brown F., Martin S. J. 1965; A new model for virus ribonucleic acid replication. Nature, London 208:861
    [Google Scholar]
  4. Brown F., Newman J. F. E., Stott E. J. 1970; Molecular weight of rhinovirus ribonucleic acid. Journal of General Virology 8:145
    [Google Scholar]
  5. Burness A. T. H., Clothier F. W. 1970; Particle weight and other biophysical properties of encephalomyo-carditis virus. Journal of GeneralVirology 6:381
    [Google Scholar]
  6. Erikson R. L. 1968; Replication of RNA viruses. Annual Review of Microbiology 22:305
    [Google Scholar]
  7. Gilbert W., Dressler D. 1968; DNA replication: the rolling circle model. Cold Spring Harbor Symposium on Quantitative Biology 33:473
    [Google Scholar]
  8. Loening U. E. 1969; The determination of the molecular weight of ribonucleic acid by polyacrylamide gel electrophoresis. Biochemical Journal 113:131
    [Google Scholar]
  9. Mcferran J. B. 1962; Bovine enteroviruses. Annals of the New York Academy of Sciences 101:436
    [Google Scholar]
  10. Macpherson L. A., Stoker M. G. P. 1962; Polyoma transformation of hamster cell clones. Virology 16:147
    [Google Scholar]
  11. Martin S. J., Brown F. 1967; Effect of virus infection on the stability and synthesis of actinomycin-resistant RNA in baby hamster kidney cells. Biochemical Journal 105:987
    [Google Scholar]
  12. Martin S. J., Johnston M. D., Clements J. B. 1970; Purification and characterization of bovine enteroviruses. Journal of General Virology 7:103
    [Google Scholar]
  13. Noble J., Kass S. J., Levintow L. 1969; Electrophoresis of poliovirus RNA. Virology 37:535
    [Google Scholar]
  14. Oberg B., Philipson L. 1967; Gel-filtration of nucleic acids on sphere condensed agarose. Archives of Biochemistry and Biophysics 119:504
    [Google Scholar]
  15. Oberg B., Philipson L. 1969; Replication of poliovirus RNA studied by gel-filtration and electrophoresis. European Journal of Biochemistry 11:305
    [Google Scholar]
  16. Peacock A. C., Dingman C. W. 1967; Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry 6:1818
    [Google Scholar]
  17. Petermann M. L., Pavlovec A. 1966; The subunits and structural ribonucleic acids of Jensen sarcoma ribosomes. Biochimica et biophysica acta 114:264
    [Google Scholar]
  18. Tannock G. A., Gibbs A. J., Cooper P. D. 1970; A re-examination of the molecular weight of poliovirus RNA. Biochemical and Biophysical Research Communications 38:298
    [Google Scholar]
  19. Wall R., Taylor M. W. 1970; Mengovirus RNA synthesis in productive and restrictive cell lines. Virology 42:78
    [Google Scholar]
  20. Wild T. F., Brown F. 1970; Replication of foot-and-mouth disease virus ribonucleic acid. Journal of General Virology 7:1
    [Google Scholar]
  21. Wild T. F., Martin S. J., Brown F. 1968; A study of the heterogeneous 37s ribonucleic acid induced by foot-and-mouth disease virus. Biochemical Journal 107:395
    [Google Scholar]
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