1887

Abstract

SUMMARY

Previous work on the serological relationships of avian influenza A viruses provided an opportunity to assess the protective effects of anti-neuraminidase anti-body in a single species system. Three viruses naturally lethal for the chicken (A/, A///63 and A///61) were selected so that two (A/ and A///63) had an antigenically similar haemagglutinin, two (A///63 and A///61) had a similar neuraminidase and two (A/ and A///61) were unrelated by surface antigens. Each virus was used to immunize a group of 7-week-old chickens using one dose of killed virus followed by one dose of live virus. Each group was then divided into three subgroups and each subgroup challenged with one of the viruses. The results of cross-challenge with live virus were assessed objectively on mortality. (1) Immunization with any of the viruses protected the birds completely against the homologous virus. (2) Immunization protected the birds completely against a heterologous virus possessing a similar haemagglutinin. (3) Immunization gave partial protection against a heterologous virus possessing a similar neuraminidase and this protection was significantly greater ( = 0.01) than unimmunized controls when birds immunized with A///63 virus were challenged with A///61. In birds immunized with A///61 and challenged with A///63 the protection was approaching significance ( < 0.1 > 0.05). The results of testing the sera from the immunized birds confirmed the seriological relationships of the viruses.

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/content/journal/jgv/10.1099/0022-1317-12-2-79
1971-08-01
2024-03-28
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References

  1. Allan W. H., Hebert C. N. 1968; The precision of virus end-point determinations. Archiv fiir diegesamte Virusforschung 25:330
    [Google Scholar]
  2. Coleman M. T., Dowdle W. R., Pereira H. G., Schild G. C., Chang W. K. 1968; The Hong Kong/68 influenza A 2 variant. Lancet 11:1384
    [Google Scholar]
  3. Compans R. W., Dimmock N. J., Meier-Ewert H. 1969; Effect of antibody to neuraminidase on the maturation and haemagglutinating activity of an influenza A2 virus. Journal of Virology 4:528
    [Google Scholar]
  4. Jahiel R. L., Kilbourne E. D. 1966; Reduction in plaque size and reduction in plaque number as differing indices of influenza virus-antibody reactions. Journal of Bacteriology 92:1521
    [Google Scholar]
  5. Kendal A. P., Madeley C. R. 1969; A comparative study of influenza neuraminidases, using automated techniques. Biochimica et biophysica acta 185:163
    [Google Scholar]
  6. Kendal A. P., Madeley C. R. 1970; Flocculation of influenza virus by specific anti-neuraminidase antibody. Archiv fiir die gesamte Virusforschung 31:219
    [Google Scholar]
  7. Kilbourne E. D., Laver W. G., Schulman J. L., Webster R. G. 1968; Antiviral activity of antiserum specific for an influenza virus neuraminidase. Journal of Virology 2:281
    [Google Scholar]
  8. Madeley C. R., Allan W. H., Kendal A. P. 1971; Studies with avian influenza A viruses: Serological relations of haemagglutinin and neuraminidase antigens of ten virus isolates. Journal of General Virology 12:69
    [Google Scholar]
  9. Meier-Ewert H., Gibbs A. J., Dimmock N. J. 1970; Studies on antigenic variation of the haemagglutinin and neuraminidase of swine influenza virus isolates. Journal of General Virology 6:409
    [Google Scholar]
  10. Schulman J. L., Khakpour M., Kilbourne E. D. 1968; Protective effects of specific immunity to viral neuraminidase on influenza virus infection in mice. Journal of Virology 2:778
    [Google Scholar]
  11. Schulman J. L., Kilbourne E. D. 1969; Independent variation in nature of haemagglutinin and neuraminidase antigens of influenza virus: distinctiveness of haemagglutinin antigen of hong kong/68 virus. Proceedings of the National Academy of Sciences of the United States of America 63:326
    [Google Scholar]
  12. Seto I. T., Chang F. S. 1969; Functional significance of sialidase during influenza virus multiplication: an electron microscope study. Journal of Virology 4:58
    [Google Scholar]
  13. Seto J. T., Rott R. 1966; Functional significance of sialidase during influenza virus multiplication. Virology 30:731
    [Google Scholar]
  14. Webster R. G., Laver W. G. 1967; Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. Journal of Immunology 99:49
    [Google Scholar]
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