1887

Abstract

SUMMARY

A virus preparation, obtained from the mycelium of , strain Q176, was purified by isopycnic centrifugation on linear gradients of potassium tartrate. Double-stranded RNA, prepared from the virus particles, revealed three components on electrophoresis on polyacrylamide gels.

The serum interferon levels in mice after intravenous injection of free virus RNA were higher and earlier than for purified virus particles, containing an equivalent amount of RNA. After reaching their maxima, the interferon activities declined at similar rates to very low levels after 45 hr for free RNA and 39 hr for virus particles.

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1971-08-01
2024-12-06
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References

  1. Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., HimmelWeit F. 1969a; Virus-Iike particles in penicillin producing strains of Penicillium chrysogenum . Nature, London 222:89
    [Google Scholar]
  2. Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., HimmelWeit F. 1969b; Penicillium CyaneO-fulvum virus and interferon stimulation. Nature, London 223:155
    [Google Scholar]
  3. Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., HimmelWeit F., Ratti G., Sharpe T. J., Planterose D. N. 1970; Antiviral activity of double-stranded RNA derived from a virus isolated from Aspergillus foetidus . Nature, London 227:505
    [Google Scholar]
  4. Banks G. T., Buck K. W., Chain E. B., HimmelWeit F., Marks J. E., Tyler J. M., Hillings M., Last F. T., Stone O. M. 1968; Viruses in fungi and interferon stimulation. Nature, London 218:542
    [Google Scholar]
  5. Bartlett G. R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234:466
    [Google Scholar]
  6. Belgian Patent 1968 No 691–881 Glaxo Laboratories Ltd
  7. Bishop D. W. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373
    [Google Scholar]
  8. Buck K. W., Kempson-Jones G. F. 1970; Three types of virus particle in Penicillium stoloniferum . Nature, London 225:945
    [Google Scholar]
  9. Cox R. A., Kanagalingam K., Sutherland E. S. 1970; Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum . Biochemical Journal 120:549
    [Google Scholar]
  10. Dunn D. B., Hall R. H. 1968; Purines, pyrimidines, nucleosides and nucleotides: physical constants and spectral properties. In Handbook of Biochemistry: Selected Data for Molecular Biology p. G3. Ed. by Sober: H. A. The Chemical Rubber Co; Cleveland Ohio:
    [Google Scholar]
  11. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419
    [Google Scholar]
  12. Franklin R. M. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proceedings of the National Academy of Sciences of the United States of America 58:782
    [Google Scholar]
  13. Fuji-Kawata I., Miura K., Fuke M. 1970; Segments of genome of viruses containing double-stranded ribonucleic acid. Journal of Molecular Biology 51:247
    [Google Scholar]
  14. Hearst J. L., Vinograd J. 1961; The net hydration of T-4 bacteriophage deoxyribonucleic acid and the effect of hydration on buoyant density behaviour in a density gradient at equilibrium in the ultracentrifuge. Proceedings of the National Academy of Sciences of the United States of America 47:1005
    [Google Scholar]
  15. Jullien P., De maeyer E. 1966; Interferon synthesis in X-irradiated animals. I. Depression of circulating interferon in C3H mice after total body irradiation. International Journal of Radiation Biology 11:567
    [Google Scholar]
  16. Kalmakoff J., Lewandowski L. J., Black D. R. 1969; Comparison of the ribonucleic subunits of reo-virus, cytoplasmic polyhedrosis vims and wound tumour virus. Journal of Virology 4:851
    [Google Scholar]
  17. Kleinschmidt W. J., Ellis L. F., Van Frank R. M., Murphy E. B. 1968; Interferon stimulation by a doublestranded RNA from a mycophage in statolon preparations. Nature, London 220:157
    [Google Scholar]
  18. Kleinschmidt W. J., Probst G. W. 1962; The nature of statolon, an antiviral agent. Antibiotics and Chemotherapy 12:298
    [Google Scholar]
  19. Knight C. A. 1963 The Chemistry of Viruses p. 82 Wien: Springer-Verlag;
    [Google Scholar]
  20. Kono Y., Ho M. 1965; The role of the reticuloendothelial system in interferon formation in the rabbit. Virology 25:162
    [Google Scholar]
  21. Lampson G. P., Tytell A. A., Field A. K., Nemes H. M., Hilleman M. R. 1967; Inducers of interferon and host resistance. I. Double-stranded RNA from extracts of Penicillium funiculosum . Proceedings of the National Academy of Sciences of the United States of America 58:782
    [Google Scholar]
  22. Lee S. H. S., Ozore R. L. 1965; Production of interferon by human mononuclear leukocytes. Proceedings of the Society for Experimental Biology and Medicine 118:190
    [Google Scholar]
  23. Lindenmann J., Gifford G. E. 1963; Studies on vaccinia virus plaque formation and its inhibition by interferon. Virology 19:302
    [Google Scholar]
  24. Lowry U. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:165
    [Google Scholar]
  25. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109
    [Google Scholar]
  26. Nemes M. M., Tytell A. A., Lampson G. P., Field A. K., Hilleman M. R. 1969; Inducers of interferon and host resistance. VII. Antiviral efficacy of double-stranded RNA of natural origin. Proceedings of the Society for Experimental Biology and Medicine 132:784
    [Google Scholar]
  27. Oellermann R. A., Els H. J., Erasmus B. J. 1970; Characterization of African horsesickness vims. Archiv fiir die gesamte Virusforschung 29:163
    [Google Scholar]
  28. Planterose D. N., Birch P. J., Pilch D. J. P., Sharpe T. J. 1970; Antiviral activity of double-stranded RNA and virus-like particles from Penicillium stoloniferum . Nature, London 227:504
    [Google Scholar]
  29. Richards E. G., Coll J. A., Gratzer W. B. 1965; Disc electrophoresis of ribonucleic acid in polyacrylamide gels. Analytical Biochemistry 12:452
    [Google Scholar]
  30. Shatkin A. J., Sipe J. D., Loh P. 1968; Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. Journal of Virology 2:986
    [Google Scholar]
  31. Smith I. 1968 Chromatographic and Electrophoretic Techniques Vol 11 p. 382 London: Heinemann;
    [Google Scholar]
  32. Smith J. D., Markham R. 1950; Chromatographic studies of nucleic acids. II. The quantitative analysis of ribonucleic acids. Biochemical Journal 46:509
    [Google Scholar]
  33. Verwoerd D. W., Louw H., Oellermann R. A. 1970; Characterization of blue tongue virus ribonucleic acid. Journal of Virology 5:1
    [Google Scholar]
  34. Wood H. A., Streissle G. 1970; Wound tumour virus; purification and fractionation of the double-stranded ribonucleic acid. Virology 40:329
    [Google Scholar]
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