The molecular size of ‘early’ and ‘late’ SV40-specific RNA synthesized in productively infected monkey kidney cells was investigated. Labelled RNA extracted from infected cells was fractionated by velocity centrifugation in a sucrose density gradient and then hybridized with excess, unlabelled SV40 DNA. The largest component in preparations of ‘early’ SV40-specific RNA, which was synthesized in actinomycin D-treated cells, had a molecular weight of approximately 1.2 × 10. Late after infection, the molecular weight of the predominant species of cytoplasmic SV40-specific RNA was about 1.7 × 10. The latter molecular size corresponds to the full transcription of one SV40 DNA strand into a polycistronic messenger RNA. The major portion of nuclear ‘late’ virus-specific RNA sedimented at 32 to 50s (molecular weight range: 2.3 to 5.7 × 10), indicating that in the nuclei of productively infected cells the virus genome can be transcribed into RNA molecules much larger than a single virus DNA strand.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error