@article{mbs:/content/journal/jgv/10.1099/0022-1317-11-3-171, author = "Kassanis, B. and Bastow, Connie", title = "Phenotypic Mixing between Strains of Tobacco Mosaic Virus", journal= "Journal of General Virology", year = "1971", volume = "11", number = "3", pages = "171-176", doi = "https://doi.org/10.1099/0022-1317-11-3-171", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-11-3-171", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Summary Strain ni 118 of tobacco mosaic virus in tobacco plants kept at 35° exists mainly as free virus RNA and insoluble virus protein but also forms a few intact virus particles. Buffer extracts of infected leaves have, therefore, very little infectivity. Similar extracts from plants inoculated with a mixture of ni 118 and the type strain tested on plants in which only ni 118 gives symptoms are very much more infective (40 to 500 times). The increase in the ni 118 infectivity of leaf extracts is even greater when the leaves are infected with the Nigerian cowpea virus instead of the type strain. The cowpea virus is a strain of tobacco mosaic virus only slightly serologically related to ni 118. Neutralization of infectivity tests, using homologous and heterologous antisera, showed that the increased infectivity of ni 118 in dual infections is caused by the nucleic acid of ni 118 being incorporated in protein of Nigerian cowpea virus.", }