1887

Abstract

Summary

The multiplication of four strains of tobacco mosaic virus was compared at temperatures of 20° to 25° and at 35° by estimating the concentration of total infective virus RNA, intact virus, virus antigen and insoluble virus protein in plants at different times after inoculation. The four strains were: the type strain, nitrous acid mutants 2 and i118, and the ‘thermophilic’ strain . The concentration of total infective RNA of all four strains reached its maximum concentration about a week after inoculation and was about ten times greater at 20° than at 35°, but the infectivity of intact virus and the virus antigen titre varied with the strain. The intact virus and virus antigen concentrations of the type strain were reduced similarly to the RNA concentration when the temperature was raised. i118 produced very little infective intact virus at 35° but as much at 20° as the type strain, although the particles were less well-formed. No intact virus of 2 was found at any temperature as the virus protein is non-functional. The strain produced about as much infective intact virus as 35° as the type strain at that temperature but much less virus at 20° than at 35°.

Virus multiplication of the four strains was affected by increased temperature in two ways: (1) the replication of the RNA of all strains was inhibited; (2) the RNA of those strains which had defective protein was degraded. Degradation was most obvious with 2, the infectivity of which, after a maximum was reached, declined at increasing rates with increasing temperature. Free RNA of i118 accumulated at 35° because the protein became insoluble but, in contrast to 2, some complete virus was produced. There was no free RNA in plants infected with type strain. The apparently greater infectivity of at 35° than at 20° resulted from the fact that its RNA is badly coated at 20°, forming unstable particles. The concentrations of total infective RNA of type strain and at 35° did not differ.

When infected plants were transferred from 35° to 20°, the infectivity of intact virus increased with the type strain and still more with i118.

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/content/journal/jgv/10.1099/0022-1317-11-3-157
1971-06-01
2024-11-12
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References

  1. Hariharasubramanian V., Zaitlan M. 1968; Temperature-induced insoluble coat protein in TMV-infected plants. Virology 36:521
    [Google Scholar]
  2. Jockusch H. 1966; Temperatursensitive Mutanten des Tabakmosaikvirus I.. In vivo-Verhalten. Zeitschrift fiir Vererbungslehre 98:320
    [Google Scholar]
  3. Jockusch H. 1968; Two mutants of tobacco mosaic virus temperature-sensitive in two different functions. Virology 35:94
    [Google Scholar]
  4. Kassanis B. 1954; Heat-therapy of virus infected plants. Annals of Applied Biology 41:470
    [Google Scholar]
  5. Kassanis B. 1957; Some effects of varying temperature on the quality and quantity of tobacco mosaic virus in infected plants. Virology 4:187
    [Google Scholar]
  6. Kassanis B., Lebeurier G. 1969; The behaviour of tomato bushy stunt virus and bromegrass mosaic virus at different temperatures in vivo and in vitro. Journal of General Virology 4:385
    [Google Scholar]
  7. Kassanis B., Mccarthy D. 1967; The quality of virus as affected by the ambient temperature. Journal of General Virology 1:425
    [Google Scholar]
  8. Kassanis B., Woods R. D. 1969; Properties of some defective strains of tobacco mosaic virus and their behaviour as affected by inhibitors during storage in sap. Annals of Applied Biology 64:213
    [Google Scholar]
  9. Lebeurier G., Hirth L. 1966; Effect of elevated temperatures on the development of two strains of tobacco mosaic virus. Virology 29:385
    [Google Scholar]
  10. Lebeurier G., Wurtz M. 1968; Propriétés d’un clone isol6 h partir d’une souche thermophile du virus de le mosaique du tabac. Compte rendu hebdomadaire des sdances de l’Acaddmie des sciences, Paris 267:871
    [Google Scholar]
  11. Sarker S., Jockusch H. 1968; Wild type and defective coat proteins of tobacco mosaic virus: electro-phoretic analysis of plant extracts in polyacrylamide gels. Biochimica et biophysica acta 106:259
    [Google Scholar]
  12. Siegel A., Zaitlan M., Sehgal O. P. 1962; The isolation of defective tobacco mosaic virus strains. Proceedings of the National Academy of Sciences of the United States of America 48:1845
    [Google Scholar]
  13. Zaitlin M., Ferris W. R. 1964; Unusual aggregation of a nonfunctional tobacco mosaic virus protein. Science, New York 143:1451
    [Google Scholar]
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