1887

Abstract

Summary

Nine strains of Newcastle disease virus were examined for their ability to inhibit cellular protein synthesis and to cause cell fusion. Inhibition of cellular protein synthesis was confined to infection with virulent strains (, , ) and the mesogenic strain . No inhibition of synthesis was recorded with avirulent strains (). Inhibition of cellular protein synthesis required virus protein synthesis within 3 hr of infection and could be inhibited by parafluorophenylalanine and Congo red. Cell fusion and haemadsorption by the various Newcastle disease virus strains also required virus-induced protein synthesis and were inhibited by cycloheximide, parafluorophenylalanine and Congo red. However, neither virus-induced inhibition of cellular protein synthesis nor cell fusion required new virus RNA synthesis, since azauridine did not affect these processes. The importance of virus-induced proteins in the inhibition of protein synthesis and cell fusion is discussed.

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/content/journal/jgv/10.1099/0022-1317-11-1-25
1971-04-01
2024-03-29
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References

  1. Allison A. C. 1967; Lysosomes in virus infected cells. Perspectives in Virology 5:29
    [Google Scholar]
  2. Allison A. C., Mallucci L. 1965; Histochemical Studies of lysosomes and lysosomal enzymes in virus-infected cell cultures.. Journal of Experimental Medicine 121:463
    [Google Scholar]
  3. Amako K., Dales S. 1967; Cytopathology of mengovirus infection. I. Relationship between cellular disintegration and virulence.. Virology 32:20l
    [Google Scholar]
  4. Bablanian R. 1970; Studies on the mechanism of vaccinia virus cytopathic effects: effect of inhibitors of RNA and protein synthesis on early virus-induced cell damage.. Journal of General Virology 6:221
    [Google Scholar]
  5. Becht H., Drzeniek R. 1968; The effect of azo dyes on myxovirus neuraminidase and on virus multiplication.. Journal of General Virology 2:261
    [Google Scholar]
  6. Bratt M. A., Gallaher W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion fromwithout by Newcastle disease virus.. Proeeedings of the National Academy of Sciences of the United States of America 64:536
    [Google Scholar]
  7. Bratt M. A., Robinson W. S. 1967; RNA synthesis in cells infected with Newcastle disease virus.. Journal of Molecular Biology 23:1
    [Google Scholar]
  8. Guskey L. E., Smith P. C., Wolef D. A. 1970; Patterns of cytopathology and lysosomal enzyme release in poliovirus-infected HEp-2 cells treated with either 2-(⍺-hydroxybenzyl)-Benzimidazole or guanidine HCl.. Journal of GeneralVirology 6:151
    [Google Scholar]
  9. Falke D. 1965; Untersuchungen über die Beziehungen zwischen Riesenzellbildung und Infektiostät von Herpes simplex virus.. Archiv für die gesomte Virusforschung 15:38
    [Google Scholar]
  10. Falke D. 1967; Ca++, Histidine and Zn++ als Faktoren bei der Riesenzellibildung durch das Herpes-virus hominis.. Zeitsehrift für Medical Mikrobiologie und Immunoforschung 153:175
    [Google Scholar]
  11. Jacobson W., Webb M. 1952; Two types of nucleic acid.. Experimental Cell Research 3:163
    [Google Scholar]
  12. Kaku H., Kamahora T. 1964; Giant cell formation in L cells infected with active vaccinia virus.. Bikenʼs Journal 6:299
    [Google Scholar]
  13. Kingsbury D. W. 1966; Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA in chick embryo cells.. Journal of Molecular Biology 18:204
    [Google Scholar]
  14. Kingsbury D.W. 1970; Replication and function of myxovirus ribonucleic acids.. Progress in Medical Virology 12:49
    [Google Scholar]
  15. Kornberg A. 1955 Lactic dehydrogenase of muscle. In Methods in Enzymology 1 Colwick S. P., Kaplan N. O. 441 New York: Academic Press;
    [Google Scholar]
  16. Long W. V., Burke D. C. 1970; The effect of infection with fowl plague virus on protein synthesis in chick embryo cells.. Journal of General Virology 6:1
    [Google Scholar]
  17. Martin E. M., Kerr I. M. 1968; Virus-induced changes in host-cell macromolecular synthesis.. Symposia of the Society for General Microbiology 18:15
    [Google Scholar]
  18. Mekler L. B., Shlyankevich M. A., Shevliaghyn V. J. 1970; Sendai virus RNA as messenger RNA determining the synthesis of early virus specific proteins.. Archiv für die gesamte Virusforschung 30:309
    [Google Scholar]
  19. Poste G. 1970; Virus-induced polykaryocytosis and the mechanism of cell fusion.. Advances in Virus Research 16:303
    [Google Scholar]
  20. Poste G., Allison A. C. 1971; The membrane fusion reaction: a theory.. Journal of Theoretical Biology.
    [Google Scholar]
  21. Reeve P., Alexander D. J. 1970; Plaque formation, cell fusion and haemadsorption by Newcastle disease virus.. Cytobios 2:55
    [Google Scholar]
  22. Reeve P., Poste G. 1971; Studies on the cytopathogenicity of Newcastle disease virus. Relation between virulence, polykaryocytosis and plaque size.. Journal of General Virology 11:17
    [Google Scholar]
  23. Reeve P., Waterson A. P. 1970; The growth cycle of avirulent strains of Newcastle disease virus.. Microbios 2:5
    [Google Scholar]
  24. Reeve P., Rosenblum M., Alexander D. J. 1970; Growth in chick chorioallantoic membranes of strains of Newcastle disease virus of differing virulence.. Journal of Hygiene Cambridge: 68:61
    [Google Scholar]
  25. Roizman B., Spear P. G. 1969; Macromolecular biosynthesis in animal cells infected with cytolytic viruses.. Current Topics in Developmental Biology 4:79
    [Google Scholar]
  26. Schäfer W., Pister L., Schneider R. 1967; Analyse des Vermehrungsmechanismus des Newcastle disease virus (NDV) mit Hilfe verschiedener Inhibitoren.. Zeitschrift für Naturforschung 22b:1319
    [Google Scholar]
  27. Thacore H., Wolef D. A. 1968; Activation of isolated lysosomes by poliovirus-infected cell extracts.. Nature London: 218:1063
    [Google Scholar]
  28. Wilson D. E. 1968; Inhibition of host cell protein and ribonucleic acid synthesis by Newcastle disease virus.. Journal of Virology 2:1
    [Google Scholar]
  29. Wilson D. E., Lo Gerfo P. 1964; Inhibition of RNA synthesis in Newcastle disease virus-infected cells by puromycin and 6-azauridine.. Journal of Bacteriology 88:1550
    [Google Scholar]
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