1887

Abstract

Summary

The cytopathic effects produced by seven strains of Newcastle disease virus grown in chick embryo cell culture were examined. The principal form of cytopathic effect involved the formation of multinucleate cells (polykaryocytes) by cell fusion. The capacity of the different Newcastle disease virus strains to induce cell fusion was related directly to their virulence for chicks and fertile eggs. The virulent (velogenic) strains , and produced significantly greater polykaryocytosis than the mesogenic strain which, in turn, produced greater polykaryocytosis than the avirulent (lentogenic) vaccine strain . The lentogenic strains and failed to produce detectable cytopathic effects. Distinct morphological differences were noted in the polykaryocytes produced by the different strains. The ability to form plaques and plaque size in chick embryo monolayers was also related to the virulence of the virus strains.

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/content/journal/jgv/10.1099/0022-1317-11-1-17
1971-04-01
2022-01-28
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References

  1. Allison A. C. 1967; Lysosomes in virus infected cells. Perspectives in Virology 5:29
    [Google Scholar]
  2. Bankowski R. A. 1964; Cytopathogenicity of Newcastle disease virus. Newcastle Disease Virus. An Evolving Pathogen231 Hanson R. P. Madison; University of Wisconsin Press:
    [Google Scholar]
  3. Bodon L., Greczi E. 1966; Acridine orange fluorescence of tissue cultures infected with Aujeskzy’s disease virus. Acta microbiologica Academiae scientiarum hungaricae 13:185
    [Google Scholar]
  4. Bratt M. G., Gallaher W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proceedings of the National Academy of Sciences of the United States of America 64:536
    [Google Scholar]
  5. Drain J. 1969; Chemistry and growth of Newcastle disease virus. Ph.D. Thesis University of London:
    [Google Scholar]
  6. Hanson R. P., Brandley C. A. 1955; Identification of vaccine strains of Newcastle disease virus. Science, New York 122:156
    [Google Scholar]
  7. Johnson C. F., Scott A. D. 1964; Cytological studies of Newcastle disease virus (NDV) in HEp-2 cells. Proceedings of the Society for Experimental Biology and Medicine 115:281
    [Google Scholar]
  8. Kohn A., Fuchs P. 1969; Cell fusion by various strains of Newcastle disease virus and their virulence. Journal of Virology 3:539
    [Google Scholar]
  9. Methods for the Examination of Poultry Biologics 1963 Publication No. 1038 National Academy of Sciences National Research Council; Washington, D.C., U.S.A.:
    [Google Scholar]
  10. Nahmias A. J., Dowdle W. R. 1968; Antigenic and biologic differences in Herpesvirus hominis. Progress in Medical Virology 10:110
    [Google Scholar]
  11. Plowrioht W. 1962; Rinderpest virus. Annals of the New York Academy of Sciences 101:548
    [Google Scholar]
  12. Poste G. 1970; Virus-induced polykaryocytosis and the mechanism of cell fusion. Advances in Virus Research 16:303
    [Google Scholar]
  13. Reeve P., Alexanoer D. J. 1970; Plaque formation, cell fusion and haemadsorption by Newcastle disease virus. Cytobios 2:55
    [Google Scholar]
  14. Reeve P., Waterson A. P. 1970; The growth cycle of avirulent strains of Newcastle disease virus. Microbios 2:5
    [Google Scholar]
  15. Reeve P., Rosenblum M., Alexander D. J. 1970; Growth in chick chorioallantoic membranes of strains of Newcastle disease virus of differing virulence. Journal of Hygiene, Cambridge 68:61
    [Google Scholar]
  16. Reeve P., Alexander D. J., Pope G., Poste G. 1971; Studies on the cytopathic effects of Newcastle disease virus. Metabolic requirements. Journal of General Virology 11:25
    [Google Scholar]
  17. Schloer G. M., Hanson R. P. 1968; Relationship of plaque size and virulence for chickens of 14 representative Newcastle disease virus strains. Journal of Virology 2:40
    [Google Scholar]
  18. Waterson A. P., Pennington T. H., Allan W. H. 1967; Virulence in Newcastle disease virus. A preliminary study. British Medical Bulletin 23:138
    [Google Scholar]
  19. Yaffe D. 1969; Cellular aspects of muscle differentiation in vitro. Current Topics in Developmental Biology 4:37
    [Google Scholar]
  20. Zuffa A., Matis J., Pleva V. 1968; Studies on several strains of pseudorabies (Aujeszky) virus showing different virulence by means of the immuno- and acridine fluorescence. Arehiv für die gesamte Virusforschung 24:396
    [Google Scholar]
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