Studies on the Assembly of Adenovirus Free

Abstract

SUMMARY

assembly of human adenovirus type 5 was studied using virus precursor materials from extracts of HeLa cells infected in Eagle’s complete and arginine-free medium. Radioisotope studies suggested that factors having assembly activities were present only in extracts of cells infected in complete medium. Extracts containing these assembly factors were added to extracts of cells infected in arginine-free medium and the mixtures, after incubation in the presence of stabilizing buffer solutions, contained particles of the same density as complete virus particles formed . The newly assembled products were DNase-resistant and had 1.5 to 2 log. greater infectivity in plaque assays than either of the two extracts alone or in unassembled mixtures. The factor(s) responsible for the assembly activity were found to be associated predominantly with the nuclear fraction of the infected cells and they were produced in greatest amounts in extracts taken at 12 to 16 hr after infection. Assembly activity of the extracts was lost when they were subjected to temperatures above 32°, trypsin treatment and fluorocarbon extraction before adding them to experimental assembly mixtures.

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/content/journal/jgv/10.1099/0022-1317-10-2-181
1971-02-01
2024-03-28
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References

  1. Borun T. W., Scharff M. D., Robbins E. 1967; Preparation of mammalian polyribosomes with the detergent Nonidet P-40. Biochimica et biophysica acta 149:302
    [Google Scholar]
  2. Caliguiri L. A., Tamm I. 1969; Membranous structures associated with translation and transcription of poliovirus RNA. Science, New York 166:885
    [Google Scholar]
  3. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167
    [Google Scholar]
  4. Hayashi K., Russell W. C. 1968; A study of the development of adenovirus antigens by the immuno-fiuorescent technique. Virology 34:470
    [Google Scholar]
  5. Horwitz M. S., Scharff M. D., Maizel J. V. 1969; Synthesis and assembly of adenovirus 2.1. Polypeptide synthesis, assembly of capsomeres, and morphogenesis of the virion. Virology 39:682
    [Google Scholar]
  6. Kushner D. J. 1969; Self-assembly of biological structures. Bacteriological Reviews 33:302
    [Google Scholar]
  7. Liebovitz A. 1963; The growth and maintenance of tissue cultures in free gas exchange with atmosphere. American Journal of Hygiene 78:173
    [Google Scholar]
  8. Mäntyjärvi R., Russell W. C. 1969; Studies on DNA synthesis in cells infected with adenovirus. Journal of General Virology 5:339
    [Google Scholar]
  9. Phillips B. A. 1969; In vitro assembly of polioviruses. I. Kinetics of the assembly of empty capsids and the role of extracts from infected cells. Virology 39:811
    [Google Scholar]
  10. Rouse H. C., Schlesinger R. W. 1967; An arginine-dependent step in the maturation of type 2 adenovirus. Virology 33:513
    [Google Scholar]
  11. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028
    [Google Scholar]
  12. Russell W. C., Knight B. E. 1967; Evidence for a new antigen within the adenovirus capsid. Journal of General Virology 1:523
    [Google Scholar]
  13. Russell W. C., Hayashi K., Sanderson P. J., Pereira H. G. 1967a; Adenovirus antigens – a study of their properties and sequential development in infection. Journal of General Virology 1:495
    [Google Scholar]
  14. Russell W. C., Valentine R. C., Pereira H. G. 1967b; The effect of heat on the anatomy of the adenovirus. Journal of General Virology 1:509
    [Google Scholar]
  15. Russell W. C., Becker Y. 1968; A maturation factor for adenovirus. Virology 35:18
    [Google Scholar]
  16. Russell W. C., Laver W. G., Sanderson P. J. 1968; Internal components of adenovirus. Nature, London 219:1127
    [Google Scholar]
  17. Russell W. C., Brodaty E., Armstrong J. A. 1970; A cytochemical study of basic proteins in adenovirus infected cells. Journal of General Virology (In the Press)
    [Google Scholar]
  18. Russell W. C., Mcintosh K., Skehel J. J. 1970; The preparation and properties of adenovirus cores. Journal of General Virology (In the Press)
    [Google Scholar]
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