Thirty-five strains of arbovirus were tested for replication in mouse embryo cell cultures; twenty-six of these multiplied and were cytopathogenic. Dengue 1 and dengue 2 may have multiplied without cytopathogenicity. Twenty-three of the viruses were also tested for the production of complement-fixing antigen; twenty-two were positive. Similar end-points were attained using standard inoculum doses in both tubes and mice for parallel titrations of selected viruses. A major advantage of mouse embryo cell cultures is their ready accessibility to all arbovirus laboratories.
BuckleyS. M.1959; Propagation, cytopathogenicity and haemagglutination-haemadsorption of some arthropod-borne viruses in tissue culture. Annals of the New York Academy of Sciences 81:172
David-WestT. S.1970b; The use of mouse embryo cell cultures in primary isolation of Chickungunya virus. Bulletin of the World Health Organisation (In the Press)
David-WestT. S.,
LabzoffskyN. A.1968; Electron microscopic studies on the development of vesicular stomatitis virus. Archiv fiir die gesamte Virusforschung 23:105
HalsteadS. B.,
SukhavachanaP.,
NisalakA.1964; Assay of mouse adapted dengue viruses in mammalian cell cultures by an interference method. Proceedings of the Society for Experimental Biology and Medicine 115:1062
KarabatsosN.,
BuckleyS. M.1967; Susceptibility of baby hamster kidney cell line (BHK21) to infection with arboviruses. American Journal of Tropical Medicine and Hygiene 16:99
LloydW.,
TheilerM.,
RicciN. I.1936; Modification of virulence of yellow fever virus by cultivation in tissues in vitro. Transactions of the Royal Society of Tropical Medicine and Hygiene 29:481
MedearisD. N.Jun,
KibrickS.1958; An evaluation of various tissues in culture for isolation of Eastern equine encephalitis virus. Proceedings of the Society for Experimental Biology and Medicine 97:152
VersteegJ.1964; Propagation and cytopathogenicity of some group A arboviruses in mouse embryo tissue culture. Archiv ftir die gesamte Virusforschung 14:332
WeinbrenM. P.1958; An improved method for the complement fixation test using small quantities of reagents. East African Virus Research Institute Report 8:38