1887

Abstract

SUMMARY

Herpes simplex virus, grown in BHK21 cells, was extensively purified by fluorocarbon treatment, ultracentrifugation, sucrose gradient centrifugation and chromatography on calcium phosphate. The purity of the product was assessed by immunodiffusion, electrophoresis in polyacrylamide gels and removal of added radioactive host material from the virus. Purified virus was disrupted with sodium dodecyl sulphate, urea and dithiothreitol, and at least eight polypeptide components were separated by electrophoresis in polyacrylamide gel.

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/content/journal/jgv/10.1099/0022-1317-10-2-163
1971-02-01
2024-12-06
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References

  1. Burness A. T. H. 1967; Separation of plaque-type variants of encephalomyocarditis virus by chromatography on calcium phosphate. Journal of Virology 1:308
    [Google Scholar]
  2. Crowle A. J. 1958; A simplified micro double-diffusion agar precipitin technique. Journal of Laboratory and Clinical Medicine 52:784
    [Google Scholar]
  3. Davis B. J. 1964; Disc electrophoresis. II. Method and application to serum proteins. Annals of the New York Academy of Sciences 121:404
    [Google Scholar]
  4. Dimmock N. J. 1969; New virus-specific antigens in cells infected with influenza virus. Virology 39:224
    [Google Scholar]
  5. Dimmock N. J., Watson D. H. 1969; Proteins specified by influenza virus in infected cells: analysis by polyacrylamide gel electrophoresis of antigens not present in the virus particle. Journal of General Virology 5:499
    [Google Scholar]
  6. Green M. 1969; Chemical composition of animal viruses. In The Biochemistry of Viruses Ed. by Levy H. B. New York: Marcel Dekker;
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  8. Olshevsky U., Becker Y. 1970; Synthesis of herpes simplex virus structural proteins in arginine deprived cells. Nature, London 226:851
    [Google Scholar]
  9. Roizman B. 1969; The herpes viruses–a biochemical definition of the group. Current Topics in Microbiology and Immunology 49:1
    [Google Scholar]
  10. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028
    [Google Scholar]
  11. Shimono H., Ben-Porat T., Kaplan A. S. 1969; Synthesis of proteins in cells infected with herpesvirus. I. Structural viral proteins. Virology 37:49
    [Google Scholar]
  12. Spear P. G., Keller J. M., Roizman B. 1970; Proteins specified by herpes simplex virus. II. Viral glycoproteins associated with cellular membranes. Journal of Virology 5:123
    [Google Scholar]
  13. Spear P. G., Roizman B. 1968; The proteins specified by herpes simplex virus. I. Time of synthesis, transfer into nuclei, and properties of proteins made in productively infected cells. Virology 36:545
    [Google Scholar]
  14. Stevens J. G., Kado-Boll G. J., Haven C. B. 1969; Changes in nuclear basic proteins during pseudorabies virus infection. Journal of Virology 3:490
    [Google Scholar]
  15. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505
    [Google Scholar]
  16. Vantsis J. T., Wildy P. 1962; Interaction of herpes virus and HeLa cells: comparison of cell killing and infective center formation. Virology 17:225
    [Google Scholar]
  17. Watson D. H. 1969; The separation of herpes virus-specific antigens by polyacrylamide gel electrophoresis. Journal of General Virology 4:151
    [Google Scholar]
  18. Watson D. H., Russell W. C., Wildy P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250
    [Google Scholar]
  19. Watson D. H., Sheddon W. I. H., Elliott A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399
    [Google Scholar]
  20. Watson D. H., Wildy P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163
    [Google Scholar]
  21. Wilcox W. C., Ginsberg H. S. 1963; Structure of type 5 adenovirus. I. Antigenic relationship of virus-structural proteins to virus-specific soluble antigens from infected cells. Journal of Experimental Medicine 118:2
    [Google Scholar]
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