1887

Abstract

SUMMARY

Herpes simplex virus, grown in BHK21 cells, was extensively purified by fluorocarbon treatment, ultracentrifugation, sucrose gradient centrifugation and chromatography on calcium phosphate. The purity of the product was assessed by immunodiffusion, electrophoresis in polyacrylamide gels and removal of added radioactive host material from the virus. Purified virus was disrupted with sodium dodecyl sulphate, urea and dithiothreitol, and at least eight polypeptide components were separated by electrophoresis in polyacrylamide gel.

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/content/journal/jgv/10.1099/0022-1317-10-2-163
1971-02-01
2021-09-24
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